中文摘要：

为进行中华补血草优良品种的保存和培育,为从分子水平进行泌盐植物耐盐机理研究奠定基础,本研究以中华补血草无菌苗叶片为外植体,对中华补血草的组织培养和再生体系进行了优化。结果表明,以MS＋6-BA 0.1 mg·L^-1＋NAA 0.2 mg·L^-1培养基为愈伤组织诱导最佳培养基,愈伤组织诱导率为100%;以MS＋6-BA 1.5 mg·L^-1＋NAA 0.2 mg·L^-1为丛生芽诱导最佳培养基,诱导率为99.06%;丛生芽继代培养适宜的培养基为MS＋NAA 0.2 mg·L^-1＋6-BA 0.15 mg·L^-1;以MS＋NAA 0.2 mg·L^-1培养基为最佳生根率培养基,生根率为87%,且根系生长良好。

英文摘要：

In order to save and breed new varieties, and to lay the foundation for studying salt tolerance mechanism of salt secreting plant by molecular techniques, tissue culture and plant regeneration of Limonium sinensewere were optimized with the leaf explants of sterile seedlings. The results showed that the optimal medium inducing the callus was MS ＋ 6-BA 0.1 mg·L^-1＋ NAA 0.2 mg·L^-1, the inducing ratio was 100%; the optimal medium of regeneration buds was MS ＋ 6-BA 1.5 mg·L^-1＋ NAA 0.2 mg·L^-1, the regeneration ratio was 99.06%; the MS medium containing NAA 0.2 mg·L^-1 and 6-BA 0.15 mg·L^-1 was optimal subculture medium;the MS medium containing NAA 0.2 mg·L^-1was the optimal medium of rooting, the rooting ratio was 87%, and the roots could have vigorous growth.