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中文摘要：
目的：建立反相离子对高效液相色谱法测定血浆吗啡及其代谢物3-葡萄糖醛酸吗啡（M3G）的浓度.方法：血浆样品以乙酸乙酯提取,以纳洛酮为内标.应用HPLC-UV方法测定吗啡浓度,分析柱Diamonsil^TM C18（250 mm×4.6 mm）,流动相为0.01 mol·L^-1磷酸二氢钾缓冲液（含0.5 mmol·L^-1的十二烷基磺酸钠）-乙腈（71：29）,检测波长210 nm,流速0.65 ml·min^1.血浆样品经β-葡萄糖醛酸苷酶水解测定其代谢物含量.结果：吗啡标准曲线线性范围宽（10-4000μg·^-1）,线性关系良好;最低检测限10μg·^-1;高、中、低浓度的回收率在85.3%以上.血浆M3G在250-8250μg·^-1浓度范围内与水解增加吗啡浓度呈线性关系.结论：本法简单,快速,血浆中杂质不干扰样品的测定,满足生物样品分析要求.
英文摘要：
Objective： To establish a reversed - phase iron - pair HPLC for determination of morphine and metabolite morphine- 3 -glucuronide （M3G） in rat plasma. Methods： Morphine was extracted from rat plasma using ethyl acetate, then determinated by HPLC - UV after separation on a DiamonsilTM Cis analytical column （250 mm × 4.6 mm ）, The mobile phase was 0.01 mol · L^-1 potassium dihvdrogen Dhosphate （0. 5 mmol·L^-1 sodium dodecvlsulfonate） - acetonitrile （71： 29）,thewavelength of detection was set at 210 nm, and flow rate was 0. 65 ml· min^-1 Internal standard was naloxone. Plasma was hydrolyzed by β -glueuronidase for determination metabolite M3G. Results： Linearity was verified from 10 to 4000 μg· L^-1, the limit of quantition（LOQ） was 10 μg· L^-1 . The recovery of morphine was above 85.3%. Linearity was also verified between the concentration of M3G （from 250 to 8250 μg· L^-1） and morphine obtained from M3G in the plasma. Conclusion：This method is simple, rapid and accurate for quantitative analysis of morphine in rat plasma.
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