中文摘要：

单核细胞诱导蛋白1（monocyte chemotactic induced protein1,MCPIP1）是近年来发现的一种重要的免疫应答负向调控蛋白。为了研究MCPIP1对A型流感病毒（influenza A virus,IAV）感染诱导的宿主抗病毒免疫应答的调控作用,我们首先证实了IAV感染A549细胞内MCPIP1的表达量显著上调。随后,在A549细胞中进行MCPIP1的过表达和表达抑制后利用Western blot方法检测了其对IAV感染诱导的细胞内NF-κB信号通路活化的影响。结果表明,过表达MCPIP1抑制了病毒诱导的NF-κB的活化,反之,抑制内源性MCPIP1的表达时则明显促进了NF-κB的激活。进一步研究发现MCPIP1可抑制NF-κB信号通路下游宿主抗病毒和促炎因子的表达,如TNF-α、IFN-β、IL-1β、IL-6等。由此推测,IAV可以通过诱导宿主MCPIP1的表达以抑制机体的抗病毒免疫应答。

英文摘要：

MCPIP1（monocyte chemotactic induced protein1）is a negative regulator controlling the stability of a set of inflammatory gene transcripts.To study the role of MCPIP1 in the antiviral immune response induced by influenza A virus（IAV）in host,we initially demonstrated that IAV infection induced the expression of MCPIP1 in IAV-infected A549 cells.Next,IAV-induced NF-κB activiation in A549 cells transfected with pcDNA-MCPIP1 or shMCPIP1plasmid was examined.The results showed that IAV-induced NF-κB activiation was inhibited by MCPIP1 overexpression and enhanced by MCPIP1 knockdown.Subsequently,inflammatory gene transcripts induced by IAV infection,such as TNF-α,IFN-β,IL-1β,IL-6,were all inhibited by MCPIP1.Taken together,we suppose a novel mechanism by which MCPIP1 expression contributes to IAV infection against the innate immune response.