中文摘要：

目的：探讨高血压对小电导型钙激活钾通道（SKCa）在大鼠阴茎海绵体中表达的影响及意义。方法：选用同龄雄性自发性高血压大鼠（SHR）及同系正常血压大鼠（WKY）各8只,10%利多卡因麻醉后连续检测海绵体内压（ICP）及平均动脉压（MAP）,并用5 V电刺激海绵体神经（CN）记录ICP/MAP值变化。运用Western blot和逆转录-聚合酶链式反应（RT-PCR）技术检测阴茎海绵体组织中SKCa蛋白和mRNA表达。结果：5 V电刺激后SHR组ICP/MAP值显著低于WKY组（0.31±0.04 vs0.76±0.06）,SKCa蛋白和mRNA的表达在SHR组（0.18±0.01、0.39±0.01）较WKY组（0.49±0.02、0.81±0.08）明显降低,差异具有统计学意义（P〈0.05）。结论：高血压可以降低大鼠勃起功能,这可能与自发性高血压大鼠阴茎海绵体组织中SKCa表达下降密切相关。

英文摘要：

Objective：To validate the effect of hypertension on small conductance calcium-activated potassium channels in cavernous tissue of spontaneously hypertensive rats. Methods： Male spontaneously hypertensive rats（SHR）（n = 8） and normotensive Wistar Kyoto rats（WKY）（n = 8） were selected. A series of electric stimulation were utilized to the corpus cavernosum nerves of rats, the changes of ICP / MAP were observed repeatedly, and the expressions of SKCa protein and mRNA were assessed by Western blotting and RT-PCR. Results： Compared with the WKY group, ICP / MAP and the expression of SKCa gene mRNA and protein were significantly lower in the SHR group（P 〈 0.05）, and the expression of SKCa protein and mRNA was significantly lower in the SHR group in comparison with that of the WKY group（P 〈 0.05）. Conclusion： Erectile dysfunction in hypertension is associated with the low expression of SKCa in the cavernous tissue of spontaneously hypertensive rats.