中文摘要：

目的：研究灵芝肽（GLP）对卡介苗与脂多糖（BCG/LPS）诱导的小鼠免疫性肝损伤的保护作用。方法：将小鼠随机分成正常组、模型组、GLP低、中、高剂量组,第1d,模型组与GLP剂量组均经尾静脉注射BCG致敏;每天灌胃一次,GLP组分别灌胃GLP60、120、180mg/kgbw,正常组、模型组给予等量生理盐水,15d后模型组和GLP组经尾静脉注射LPS攻击,诱导小鼠肝损伤。通过脏器重量和体重计算肝、脾脏指数;用试剂盒的方法分别检测各组小鼠血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）活力,肝组织中丙二醛（MDA）、谷胱甘肽（GSH）、一氧化氮（NO）含量及超氧化物歧化酶（SOD）与谷胱甘肽过氧化物酶（GSH-Px）的活力;并进行肝组织病理学镜检。结果：GLP高剂量组的肝、脾指数与血清ALT、AST活性均极显著地低于模型组（p〈0.01）,各种剂量的GLP均可显著地对抗BCG/LPS诱导的小鼠肝损伤所致的肝组织中MDA、NO含量的升高以及肝组织中SOD、GSH-Px活力与GSH含量的降低（p〈0.05～0.01）;肝组织病理学形态逐步好转;尤其以180mg/kgbw给予GLP,防止肝损伤的效果最佳,趋近于正常组。结论：GLP对BCG/LPS诱导的小鼠免疫性肝损伤有很好的保护作用。

英文摘要：

Objective： The hepatoprotective effects of Ganoderma lucidum peptides （GLP） were researched on Bacille- Calmette- Gu＇erin and lipopolysaccharide （BCG/LPS）- induced immunological liver injury in mice. Method： Mice were randomly divided into five groups, namely normal, model and three GLP-treated groups. The mice from model group and GLP- treated groups were treated with BCG from tail vein （iv） on the ftrst day. GLP-treated groups were daily given （ig） the doses of 60, 120 and 180 mg GLP/kg bw respectively. Normal and model groups simultaneously were aiven the same amount of physiological saline for 15 days. Then the mice from model group and GLP-treated groups were treated with LPS （iv）. The index of liver and spleen were calculated. AST/ALT activities in serum, MDA, GSH and NO levels and SOD/GSH-Px activity in liver were assayed by the detection kits according to the manufacturer＇s protocols. Liver histopathological charactersistics were observed under fight microscopy. Results： The index of fiver and spleen in mice and ALT/AST activities in serum from the group at high dose are significantly lower than model group＇s （p 〈 0.01）. GLP at all of the doses significantly inhibits NO/MDA lever in liver and reduces of SOD/GSH-Px activity and GSH content in fiver （p 〈 0.05-0.01）. The fiver histopathological characteristics gradually straighten up. At the dose of 180 mg/kg bw GLP, the hepatoprotective effect is the best and the index is close to normal group＇s. Conclusion： GLP possesses potent hepatoprotective effects against BCG/LPS-induced fiver damage in mice.