中文摘要：

目的研究昆明小鼠对不同剂量可卡因的敏感度，为昆明小鼠在神经科学领域的应用，制别是可卡因成瘾的研究提供参考。方法同龄（8～12周）的雄性昆明小鼠，分别腹腔注射10mg／kg、2qmg／kg的可卡因，条件位置偏爱实验（Conditioned place preference）和行为敏化实验（Behavior sensitization检NA,鼠行为学变化，观察昆明小鼠在不同剂量可卡因刺激下的行为学变化，以此来确定昆明小鼠对不每剂量可卡因的敏感度。结果在条件位置偏爱实验中，可卡因20mg／kg剂量组（N=11）与可卡因10mg／kg剂量组（N=12）相比，第9天与第2天在白箱中的时间差score（post-pre）有统计学差异（P〈0．05）；而在行为敏化实验中，可卡因20mg／kg组（N=8）与可卡因1Omg／kg组（N=12）相比，自主活动次数明显增加，蓓统计学差异（P〈0．05）；在第16天给予一针与之前同剂量的可卡因，可卡因20mg／kg组与可卡因1Omg／kg组相比，自主活动次数有统计学差异（P〈0．05）。结论昆明小鼠在20mg/kg可卡因的刺激下，能产生要为明显的条件位置偏爱以及更加强烈的行为敏化反应，是较为理想的可卡因成瘾小鼠模型。

英文摘要：

Objective To investioate the sensitivity ofKunmin9 mice to different doses of cocaine, and provide reference for the cocaine related research of Kunmin9 mice. Methods Kunmino mice, male, aoed 8-12 weeks, were used. They were randomly divided into two groups. One 9roup was injected with 10 mg/kg cocaine, while the other 20 mg/kg cocaine. Conditioned place preference and Behavior sensitization were used to measure the behavioral differences before and after cocaine treatment. Results In the conditioned place preference analysis, the time difference in the white box on day 9 and day 2 （post-pre） was statistically siGnificant （P〈0.05） between the two groups. In the Behavior sensitization test, the ambulatory accounts between the two 9roups were found to be statistically siGnificant（P〈0.001 ）durin9 the time treated with cocaine. And then they were put back to the colony room for 1 week before they received a challenoino dose of cocaine. On the challenoin9 day, the ambulatory accounts were found to be statistically siGnificant between them （P=0.001）. Conclusion Kunmin9 mice can produce more obvious conditioned place preference and more intense behavioral sensitization reaction when treated with 20 mg/kg cocaine.