中文摘要：

为了研究胃癌细胞中幽门螺杆菌（Hp）毒素蛋白CagA诱导的蛋白差异表达及其基因在人胃癌组织中的表达,用Hp感染胃癌细胞系SGC-7901和AGS及用含CagA基因的表达载体稳定转染SGC-7901细胞,构建3组实验模型.提取各组细胞的总蛋白进行双向凝胶电泳,筛选3组重叠的差异表达蛋白质斑点进行质谱鉴定.共获得135个差异表达的蛋白质,其中上调蛋白质73个,下调蛋白质62个.鉴定出10个差异表达蛋白质,其中有6个差异表达蛋白是首次发现,它们主要参与细胞的能量代谢和信号转导等.最后定量检测了这10个差异表达蛋白基因在人胃癌组织中的表达,发现有4个基因高表达和1个基因低表达.本结果将为研究幽门螺杆菌感染引起胃癌的分子机制提供新的线索.

英文摘要：

To study the differential expression of the proteins induced by CagA in gastric cancer cells and their gene expression in human gastric cancer tissues,human gastric cancer cell lines SGC-7901 and AGS cells were infected with Helicobacter pylori（Hp）.SGC-7901 cells were stably transfected with the vector pcDNA3.1ZEO（-） /CagA,carrying the full-length CagA gene,to establish three experimental models.Total proteins were extracted and then loaded on two-dimensional electrophoresis（2-DE）.The differently expressed protein spots overlapped among three models were selected and identified by LC-MS/MS.One hundred thirty-five of the differential proteins were obtained in three models,in which 73 proteins were up-regulated and 62 proteins were down-regulated.Ten of the proteins,including lactate dehydrogenase,calmodulin,RanGTPase-activating protein,P43 protein etc,were identified.These proteins are involved in different functions,such as energy metabolism,cytoskeleton and signal transduction and others.Finally,the real-time RT-PCR was further used to detect the gene expression of these differential proteins in human gastric cancer tissues.The results showed that the genes of β-actin,lactate dehydrogenase,pre-mRNA processing factor 19 and calmodulin were up-regulated and the gene of Ran-specific GTPase-activating protein was down-regulated.These results will provide a new clue for studying the molecular pathogenesis of gastric cancer with Hp infection.