中文摘要：

研究了在超临界CO2介质中非离子表面活性剂对胰酶催化牛血清白蛋白(BSA)水解的影响,通过系统水含量及系统压力的变化来分析四种非离子表面活性剂平平加O、十三烷基醇聚氧乙烯醚(1380)、聚乙二醇辛基苯基醚(OP-10)及Tween 80对胰酶催化牛血清白蛋白水解的影响,并用SDS-PAGE凝胶电泳法及分析软件TotalLab对蛋白质的水解度进行分析。研究表明,非离子表面活性剂在低系统压力及低系统水含量下能促进胰酶催化牛血清白蛋白水解,使水解率提高20%以上,且水解率随着系统压力的升高而上升,随着系统含水量的升高而下降。当系统水含量达到30μL以上时,非离子表面活性剂对胰酶催化牛血清白蛋白水解的影响不明显。

英文摘要：

This paper studied about the influence of nonionic surfanctants on hydrolysis degree of bovine serum albumin(BSA) catalyzed by pancrease in supercritical CO2.The four kinds of nonionic surfactants include pergal O,1380,OP-10 and Tween 80,and the influence of hydrolysis degree of BSA was analyzed by changing systematic content of water and systematic pressure.Using polyacrylamide gel electrophresis(SDS-PAGE) and a kind of analysis software Total Lab,the results indicated that nonionic surfactants could promo...