中文摘要：

EMF2基因是植物营养组织中的主要开花抑制基因,在植物花器官发育过程中发挥重要的作用。为给油茶性状表观遗传学调控研究提供参考依据,以＂华鑫＂油茶的组培幼苗为试验材料,采用3′RACE和5′RACE技术对油茶EMF2基因进行克隆及序列分析。结果表明：该基因全长2 210 bp,其中开放阅读框为1 866 bp,编码621个氨基酸,该基因被命名为Co-EMF2。多序列比对后发现,Co-EMF2具有EMF2基因特有的保守序列和相关特征,且与其它植物的EMF2基因具有较高的相似性。文中进而对其蛋白质序列和理化性质进行了分析,结果表明：其蛋白质属不稳定蛋白,等电点为6.17,是疏水性蛋白,亚细胞定位预测结果表明其位于细胞核中。

英文摘要：

EMF2 gene maintains vegetative development by repressing flowering program, and plays an important role in the process of flower organ development. In order to offer a reference for the researches on epigenetic regulation of Camellia oleifera characters, the full-length eDNA of EMF2 gene in C. oleifera （Huaxin） was cloned and analyzed by 5＇RACE and 3＇RACE techniques. The results show that the full length of the gene is 2 210 bp, the ORF box included 1 866 bp and encodes 621 amino acids, and it is named Co-EMF2. The results of multiple sequence alignment show that Co-EMF2 has the typical conserved sequences of EMF2 and relevant characters, and it has high homology with EMF2 in other plant species, the protein is a labile and hydrophobic nuclear protein, and its lsoelectric point is 6.17.