中文摘要：

目的应用实时定量聚合酶链反应（Real—timePCR）技术检测胰岛素生长因子。1（IGF-1）mRNA在糖尿病大鼠膀胱的表达变化。方法将30只SD雄性大鼠随机分成3组，正常对照组10只，糖尿病组10只，治疗组10只。糖尿病组、治疗组大鼠按60mg／kg腹腔注射链脲佐菌素，72h后测空腹尾静脉血葡萄糖浓度，血糖浓度〉16．7mmol／L为糖尿病大鼠造模成功。治疗组SD大鼠给予皮下注射精蛋白锌胰岛素，使血糖维持在3．9～10．0mmol／L。8周后取大鼠膀胱，提取RNA后应用Real—time PCR方法检测IGF-1 mRNA的△Ct值。结果糖尿病组IGF-1mRNA△Ct值为6．141±0．916，正常对照组为4．018±0．491，治疗组为4．922±0．788。ACt值越低，实际拷贝数越高，基因表达量越高。IGF-1 mRNA在糖尿病组表达量明显下调（P〈0．05）。经胰岛素治疗后可上升（P〈0．05）。结论IGF-1 mRNA在糖尿病大鼠膀胱中表达明显下调；经胰岛素治疗后，IGF-1 mRNA在糖尿病大鼠膀胱中的表达升高。

英文摘要：

Objective To observe the insulin-like growth factor-1 （IGF-1） mRNA expression in diabetic rat bladder. Methods Thirty Sprague-Dawley （ SD ） male rats were randomly divided into 3 groups： normal control group, diabetic group, and treatment group,n = 10 each. The rats in diabetic group and treatment group were injected intraperitoneally with 60 mg/kg Stretopzin （STZ）. After 72 h, fasting blood glucose was measure, and BG 〉 16. 7 mmol/L indicated the successful establishment of diabetic model. SD rats in treatment group were given subcutaneous injection of protamine zinc insulin, so BG remained at 3.9-10. 0 mmol/L. Bladders of three groups were obtained after 8 weeks, The mRNA from bladders was extracted for detection of △Ct of IGF-1 mRNA by real-time quantitative polymerase chain reaction （Real-time PCR）. Results IGF-1 mRNA A Ct in the diabetic group, normal control group and treatment group was 6. 141 ± 0. 916, 4. 018±0. 491 and 4. 922 ± 0. 788. the lower the Deha Ct value was lower, the greater the actual copy number was and the higher the amount of gene expression. Conclusion IGF-1 mRNA expression in diabetic rat bladder was significantly reduced. The insulin treatment may up-regulate the IGF-1 mRNA expression in the bladders of diabetic rats.