中文摘要：

背景 Glioma 干细胞(GSC ) 假设安置那在 gliomas 以内的房间的 subpopulation 有真 clonogenic 和 tumorigenic 潜力。显著地，到 GSC 的更争论的相互关联是处于不同文化条件的房间可能显示不同干细胞性质。就这些可能性而言，我们使用了在不同文化 conditions.Methods C6 细胞下面比较 C6 glioma 细胞的干细胞特征的一条途径在三个不同生长条件下面是有教养的，即，支持者生长在常规 10 浆液媒介，在没有浆液的媒介的非支持者范围生长，象在没有浆液的媒介的laminin涂的烧瓶上的支持者生长一样。生长特征通过 neurosphere 形成试金和房间周期分析对比地被检测。标记被 immunofluorescence，亲戚量的反向的抄写(RT )-PCR,Western 弄污和流动 cytometry 决定。方面人口房间经由流动 cytometry 被分析。肿瘤模型被磁性的回声成像和 hematoxylin 与曙红染色检测。数据分析与软件(17.0 ).Results C6 房间(C6-Adh， C6-SC-Sph 和 C6-SC-Adh ) 显示出的社会科学统计套装软体被执行到推迟 C6-SC-Sph，支持者 C6-Adh 和 C6-SC-Adh 的特殊生长模式和增长 capacity.Compared 显示了更高的生长比率。C6-SC-Sph 和 C6-SC-Adh 显示出 neurosphere 形成和自强的提高的能力。高方面人口比率在 C6-SC-Sph 和 C6-SC-Adh 被检测。CD133 没在所有三种房间被检测。相反地， Nestin 并且 -- 导管素被表明积极，尽管如此没有统计意义(P 0.05 ) 。有趣地，降低 glial fibrillary 的表示酸的蛋白质在 C6-SC-Sph 和 C6-SC-Adh 被表明。C6-Adh， C6-SC-Sph 和 C6-SC-Adh 都在 situ oncogenicity 被显示，当幸存时间的统计差别不是 confirmed.Conclusions C6 glioma 时当转了进干细胞文化状况时，房间线被赋予以能中等在 vitro 被充实的一些 GSC 显型。结果的肿瘤范围可以不是一个前提或 GSC 的健全来源，在干细胞媒介的支持者文化不是赞成在 vivo 膨胀的 GSC 的一个生长条件。

英文摘要：

Background Glioma stem cell （GSC） hypothesis posits that a subpopulation of cells within gliomas have true clonogenic and tumorigenic potential. Significantly, a more controversial correlate to GSC is that cells in different culture conditions might display distinct stem cell properties. Considering these possibilities, we applied an approach comparing stem cell characteristics of C6 glioma cells under different culture conditions.Methods C6 cells were cultured under three different growth conditions, i.e., adherent growth in conventional 10% serum medium, non-adherent spheres growth in serum-free medium, as well as adherent growth on laminin-coated flask in serum-free medium. Growth characteristics were detected contrastively through neurosphere formation assay and cell cycle analysis. Markers were determined by immunofluorescence, relative-quantitative reverse transcription （RT）-PCR,Western blotting and flow cytometry. Side population cells were analyzed via flow cytometry. Tumor models were detected by magnetic resonance imaging and hematoxylin ＆ eosin staining. Data analyses were performed with SPSS software （17.0）.Results C6 cells （C6-Adh, C6-SC-Sph and C6-SC-Adh） showed distinctive growth patterns and proliferation capacity.Compared to suspending C6-SC-Sph, adherent C6-Adh and C6-SC-Adh displayed higher growth ratio. C6-SC-Sph and C6-SC-Adh showed enhanced capability of neurosphere formation and self-renewal. High side population ratio was detected in C6-SC-Sph and C6-SC-Adh. CD133 was not detected in all three kinds of cells. Conversely, Nestin and β-Ⅲ-tubulin were demonstrated positive, nonetheless with no statistical significance （P 〉0.05）. Interestingly, lower expression of glial fibrillary acidic protein was demonstrated in C6-SC-Sph and C6-SC-Adh. C6-Adh, C6-SC-Sph and C6-SC-Adh were all displayed in situ oncogenicity, while statistical difference of survival time was not confirmed.Conclusions C6 glioma cell line is endowed with some GSC phenotypes that can be moderately enr