中文摘要：

为了探索肥大细胞（MC）钙信号系统在肉鸡肺动脉高压综合征（PHS）形成中的作用,采用低温诱发肉鸡PHS,给予钙通道阻断剂（维拉帕米）后,右心导管法测定肉鸡血流动力学,常规阿尔新蓝法显示MC,免疫组化方法显示组胺,利用焦锑酸钾组化电镜技术,观察肺组织MC脱颗粒时胞浆钙沉积现象。结果：（1）维拉帕米组肉鸡肺动脉收缩压和舒张压显著低于低温组;（2）维拉帕米维持右心舒缩功能相对正常,抑制右心肥大;（3）维拉帕米组肺血管壁MC数显著（P〈0.05）高于低温组,心脏MC数极显著（P〈0.01）高于低温组,31和38日龄维拉帕米组肺呼吸毛细管和肺小动脉血管壁MC脱颗粒率也显著（P〈0.05）低于低温组;（4）维拉帕米组肺呼吸毛细管组胺阳性细胞数显著（P〈0.05）高于低温组,血管壁组胺阳性细胞数亦显著（P〈0.05）高于低温组;（5）肺动脉高压综合征肉鸡的脱颗粒MC胞浆中可见Ca^2＋颗粒沉积,出现Ca^2＋超载现象。研究表明钙信号参与PHS肉鸡MC的激活,维拉帕米稳定肺和心脏中的MC,防止MC脱颗粒,抑制组胺的释放,同时降低了肺动脉压,这可能是维拉帕米抑制PHS形成的重要机制之一。

英文摘要：

The effect of Ca^2＋ channel of mast cells （MC） on the development of pulmonary hypertension syndrome （PHS） was investigated in broiler chickens. The birds with PHS were induced by low ambient temperature. The pulmonary artery pressure was measured using right cardiac catheter. MC were stained with Alicience blue. Histamine was shown using immunohistochemistry. The deposit of Ca^2＋ was observed in MC by transmission electron micrograph. The results showed： （1） The Ca^2＋ channel blocking agent （verapamil） can decrease the pulmonary artery systolic pressure and pulmonary artery diastolic pressure significantly. （2） Verapamil decreased the right ventricular pressure and ascites heart index（P〈0.05）. （3） Verapamil promoted the number of MC in peripheral pulmonary blood vessel walls and right ventricle（P〈0.05）, and markedly decreased（P〈0. 05） the ratio of degranulation of MC. （4） Verapamil increased the number of histamine positive cells in the lung（P〈0. 05）. （5） Intracellular Ca^2＋ was increased significantly in degraunlated MC. These results suggested that verapamil inhibited the degranulation of MC and releasing mediators to prevent the development of the PHS.