中文摘要：

瞄准：在开发期间在老鼠胰调查 paxillin 的表达式和本地化。方法：从 Sprague Dawley 老鼠胎儿，胚胎，小动物，和成年动物的 Pancreata 在这研究被使用。在不同发展阶段的 pancreata 的 paxillin 的表示层次被反向的抄写聚合酶链反应并且西方的弄污检测。为了在发展中的老鼠胰， immunohistochemistry 和 double-immunofluorescent 识别 paxillin 的房间地点，染色分别地为特定的房间标记和 paxillin 用抗体被执行。结果：最高的 paxillin mRNA 水平在在以后发展的时期跟随减少的 E15.5 (胚胎白天 15.5 ) 被检测(P < 0.05 对 E18.5， P0 和成年人，分别地) ，并且通过到成年人的从 E15.5 的转变的日益增多地增加的 paxillin 蛋白质表情被检测。paxillin 积极染色在在在胰开发期间测试的每个阶段的 Langerhans 的老鼠小岛主要是局部性的。结论：在从不同阶段的老鼠胰的 paxillin 的动态表达式显示 paxillin 可能涉及胰腺的开发的一些方面。

英文摘要：

AIM： To investigate the expression and localization of paxillin in rat pancreas during development.METHODS： Pancreata from Sprague Dawley rat fe- tuses, embryos, young animals, and adult animals were used in this study. Expression levels of paxillin in pan- creata of different development stages were detected by reverse transcription polymerase chain reaction and Western blotting. To identify the cell location of paxillin in the developing rat pancreas, immunohistochemistry and double-immunofluorescent staining were per- formed using antibodies for specific cell markers and paxillin, respectively.RESULTS： The highest paxillin mRNA level was detected at E15.5 （embryo day 15.5） following a decrease in the later developmental periods （P 〈 0.05 vs E18.5, P0and adult, respectively）, and a progressively increased paxillin protein expression through the transition from E15.5 to adult was detected. The paxillin positive stain- ing was mainly localized in rat islets of Langerhans at each stage tested during pancreas development.CONCLUSION： The dynamic expression of paxillin in rat pancreas from different stages indicates that paxillin might be involved in some aspects of pancreatic devel- opment,