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中文摘要：
以早期从海洋环境中分离得到的38株海洋真菌为材料，采用苏丹黑染色法初筛，摇瓶法复筛，得到1株油脂产率相对较高（27．96％）的菌株MD2，经鉴定，该菌株为粘红酵母（Rhodotorulaglutinis）．再以MD2为出发菌株，经紫外诱变、亚硝基胍诱变和紫外-亚硝基胍复合诱变，最终得到突变菌株MD2-1；采用酸热法提取粘红酵母MD2-1的菌体油脂，测定了其产脂能力，并采用气相色谱-质谱联用法（GC-MS）测定其脂肪酸组成．结果表明：MD2-1的产脂能力较强，油脂质量达菌体干质量的45．32％，较MD2提高17．36％；MD2-1菌体油脂中检测出9种脂肪酸，其脂肪酸组成与植物油近似，其中10十八（碳）烯酸占脂肪酸总量的71.53％，为10-十八（碳）烯酸工业化生产提供菌种资源，为其规模化生产奠定基础．
英文摘要：
Thirty-eight marine fungi strains were isolated from marine environment and subjected to preliminary screening with Su dan Black dye and further screening by shake-flask culture. As a result, strain MD2 was found to have the highest lipid content of 27.96% and identified as Rhodotorula glutinis. The strain was mutagenized respectively with UV light, nitrosoguanidine （NTG） and their combination, obtaining the mutant MD2-1. The lipid was extracted from the strain Rhodotorula glutinis MD2-1 by acid- heat extracting methods, and its fatty acids was analyzed by GC-MS. The results showed that R. glutinis MD2-1exhibited strong a- bility to produce lipid, and the total amount of lipid represented an average of 45.32% of the dry strain weight. The fatty acid com- position of the lipid from R. glutinis MD2-1 was similar to vegetable oil and fat, and the 10-octadecenoic acid accounted for 71.53 % of the total fatty acids, so this research made a major significance： provided seed resources for industrial production of 10-octadeceno ic acid and laid the foundation for its large-scale production.
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