中文摘要：

研究顺式-阿霍烯（Z—Ajo）和反式-阿霍烯（E—Ajo）的肠细胞摄取、转运和外排特性。采用体外培养的人结肠Caco-2细胞单层模型评价，应用高效液相色谱法测定Z—Ajo和E—Ajo的含量。结果表明，仅能在Caco-2细胞单层的顶侧检测到Z—Ajo或E—Ajo；阿霍烯在Caco-2细胞中的代谢可被抗氧化剂维生素C、细胞色素P450药物代谢酶3A亚型抑制剂甲吡酮和ATP抑制剂叠氮化钠所抑制。Z—Ajo和E—Ajo皆不能透过Caco-2单层细胞而被迅速代谢，其代谢与CYP450药物代谢酶有关。

英文摘要：

The characteristics of uptake, transepithelial transport and efflux of Z- and E-ajoenes isolated from the bulbs of Allium sativum were studied. A human colon cell model Caco-2 cell monolayers in vitro cultured had been applied to study the characteristics of uptake, transepithelial transport and efflux of Z- and E-ajoenes. The quantitative determination of Z- and E-ajoenes was performed by highperformance liquid chromatography. Z- and E-Ajoenes can be detected only in the apical side and can be metabolized, but both compounds can not be transported from apical-to-basolateral and basolateral-to- apical directions in cultured Caco-2 cell monolayers. The metabolism of Z- and E-ajoenes in Caco-2 cell monolayers can be partially inhibited by vitamin C as an anti-oxidant, metyrapone as an inhibitor to subtype CYP3A of cytochrome P450 drug metabolism enzymes, and sodium azide as an inhibitor to ATP production. It is shown that neither Z-ajoene nor E-ajoene can pass through Caco-2 cell monolayers, and that they can be metabolized by the cells. The metabolism might be in correlation with cytochrome P450 drugs metabolism enzymes in Caco-2 cell monolayers.