中文摘要：

研究酪蛋白糖巨肽（CGMP）对正常小鼠肠相关淋巴组织的免疫应答反应的影响,对阐述CGMP的生物学功能具有重要意义。本实验将BALB/c雌性小鼠28只随机分为4组,每组7只,分别为0.9%生理盐水对照组（0.2mL/d）,低、中、高3种剂量组（30、120、300μg/d）。连续灌胃16d后,取脾脏、PP结（peyers patches）和肠系膜淋巴结（mesenteric lymph node,MLN）,制备细胞悬液,进行三色荧光标记后,用流式细胞仪检测CD3＋、CD3＋CD4＋和CD3＋CD8＋T淋巴细胞亚群的变化。结果表明：CGMP各剂量组均能够促进脾脏和PP结中CD3＋和CD3＋CD4＋细胞的显著增加（P〈0.05）,高剂量能引起脾脏CD3＋CD8＋淋巴细胞的显著增多（P〈0.05）,低剂量能引起脾脏CD3＋CD4＋/CD3＋CD8＋的显著增加（P〈0.05）;低剂量CGMP能使MLN中CD3＋CD8＋淋巴细胞显著增减少（P〈0.01）。研究证实长期灌胃CGMP会诱导肠黏膜产生获得性的免疫应答,并且不会引起机体产生口服耐受。,

英文摘要：

In order to explore the effect of casein-glycomacropeptide（CGMP） on the intestinal mucosal immune response in normal mice,28 female BALB/c mice were randomly divided into 4 groups for 7 mice in each group and by gavage given physiological saline（as control group） or CGMP at dosages of 30,120μg/d and 300μg/d（low-,medium-and high-dosage groups） for 16 consecutive days.After treatment,all the mice were sacrificed and their spleen,peyer＇s patches and mesenteric lymph node（MLN） were collected to examine the change of CD3＋,CD3＋CD4＋ and CD3＋CD8＋T lymphocytes subpopulations using flow cytometry.The results indicated that CGMP resulted in a significant increase in CD3＋ and CD3＋CD4＋ lymphocytes in spleen and peyer＇s patches（P 0.05） in a dosage-dependent manner.Compared with the control group,high-dosage CGMP administration resulted in a higher percentage of CD3＋CD8＋ lymphocytes in spleen（P 0.05）,and low-dosage CGMP administration made the CD3＋CD4＋/CD3＋CD8＋ ratio significantly（P 0.05） increase but the percentage of CD3＋CD8＋ lymphocytes in MLN significantly decrease（P0.01）.The above results demonstrate that long-term gastric perfusion of CGMP can induce intestinal mucosal adaptive immune response without tolerance to its oral administration.