结合DNA酶优异的氧化还原催化特性和碳纳米管的电化学特性,制备了单壁碳纳米管一DNA酶复合材料,并通过壳聚糖将其固定到玻碳电极表面构建了电化学生物传感界面.研究了单壁碳纳米管.DNA酶复合结构的氧化还原反应催化特性,并以此为传感平台构建了葡萄糖氧化酶电化学生物传感器.结果表明,单壁碳纳米管一DNA酶复合材料修饰的电极对过氧化氢的响应具有较宽的线性范围(5×10^-6~1×10^-2mol/L)和良好的检测灵敏度(检出限为1×10^-6mol/L).采用制备的葡萄糖氧化酶传感器实现了对葡萄糖的快速灵敏检测.
Taking advantage of the excellent catalytic redox activity andthe electrochemical property of DNAzyme and carbon nanotubes, a novel nano-eomplex of single-walled carbon nanotubes (SWNTs)/ DNAzyme was prepared, and the obtained nano-eomplex was finally immobilized on the surface of glass carbon electrode (GCE) with the assistance of chitosan to fabricate electrochemical biosensing interface. The catalytic property of the SWNTs/DNAzyme complex was explored, and a glucose oxidase biosensor was then prepared based on the fabricated electrochemical biosensing platform. The SWNTs/DNAzyme complex-based biosensing platform can direct response to hydrogen peroxide at low potential of -0.24 V with high sensitivity (the detection limit is 1×10^-6 mol/L) and wide linear range(5×10^-6--1×10^-2 mol/L), and the prepared glucose oxidase biosensor was used to fast and sensitive detection of glucose.