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中文摘要：
目的：探讨β2肾上腺素受体（β2-AR）在皮肤创伤修复上皮间质化（EMT）过程中的作用机制及意义。方法：采用免疫印迹法（Westernblot）、逆转录一聚合酶链式反应（RT-PCR）、免疫荧光技术等实验方法，体外观察β2-AR激动剂（ISO）和抑制剂（ICI）对上皮细胞间质化标志物（a-平滑肌肌动蛋白、波形蛋白、snail）表达的影响及创面皮肤上皮化标志物（E-钙黏附素）表达的变化。结果：β2-AR可通过介导皮肤创面愈合的EMT过程，促进表皮细胞向间质表型转化，且运动和迁移能力在该过程中不断增强。结论：β2-AR是皮肤上皮间质化的关键分子，其介导的EMT过程是皮肤创面愈合的生物学基础。
英文摘要：
Objective： To explore the mechanism and significance of β2-adrenergic receptor （β2-AR） in the procedure of epithelial mesenchymal transition （EMT） during wound healing and tissue regeneration. Methods： The influence of β2-AR agonist （ISO） and inhibitor （ICI） on the expression of markers of mesenchymal transition （a-smooth muscle actin, vimentin, snail） or marker of epithelization （E-cadherin） in epidermis cells during wound healing and tissue regeneration was observed using western blot, reverse transcription polymerase chain reaction （RT-PCR） and immunofluorescence. Results： The epithelial cells gained mesenchymal phenotypic feature, and got stronger ability in movement and migration. β2-AR was a critical molecule which mediated EMT process. Conelusions： β2-AR is a key molecule in the procedure of EMT, and β2-AR mediated EMT is a biological basis of skin wound healing and matrix remodeling.
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