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六种白腐菌预处理对毛白杨木材酶水解效果的影响
  • ISSN号:1672-6472
  • 期刊名称:《菌物学报》
  • 时间:0
  • 分类:TS743.9[轻工技术与工程—制浆造纸工程]
  • 作者机构:[1]北京林业大学微生物研究所,北京100083, [2]北京林业大学水土保持学院,北京100083
  • 相关基金:教育部新世纪优秀人才支持计划;国家自然科学基金(No.30900006)
中文摘要:

通过化学分析和酶水解试验,研究了不同的白腐菌对毛白杨的预处理效果及不同组分的降解对酶水解的影响。毛白杨木片经6种白腐菌预处理30d后,各组分都发生了降解,其中半纤维素的损失最为显著,Trametes ochracga C6888引起半纤维素降解率高达47.19%,其次是纤维素和酸不溶木素的降解。在后续酶水解过程中,6种白腐菌处理后的样品显示出不同的水解模式,菌株Trametes ochraceaC6888、rpubescensC7571和T.versicolor C6915预处理效果最为显著,还原糖得率在整个酶水解过程中一直高于对照,其中T.ochracea C6888在水解96h后还原糖得率达到15.93%,比未处理样品提高了25%。分析酸不溶木素降解率及半纤维素降解率与还原糖得率的关系发现,不同菌株在作用同一种基质时,预处理效果差异显著,木质素和半纤维素的脱除都会影响木质纤维素的酶水解。

英文摘要:

The pretreatment efficiency of different fungi and the effects of different components on enzymatic hydrolysis were investigated through chemical analysis and enzymatic hydrolysis experiments. Chemical components of Populus tomentosa were all degraded after pretreatment by six species of white rot fungi for 30 days. Hemicellulose loss was found to be the most significant, and 47.19% of the loss was caused by Trarnetes ochracea C6888. The losses of cellulose and acid insoluble lignin were less than those of hemicellulose. The samples treated by six species of white rot fungi displayed different patterns in the subsequent enzymatic hydrolysis. Reducing sugar yields were higher in the samples treated by Trametes ochracea C6888, T. pubescens C7571 and T. versicolor C6915 during the whole of the hydrolysis periods. 15.93% of reducing sugar yield was achieved by T. ochracea C6888 in 96h, increasing by 25% as compared with the untreated sample. The relationships between the loss of acid insoluble lignin and the yield of reducing sugar, and hemicellulose loss and reducing sugar yield were investigated. It was found that different fungi varied greatly during pretreatment with the same substrate, and the losses of lignin and hemicellulose could influence on the enzymatic hydrolysis of lignocellulose.

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期刊信息
  • 《菌物学报》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学院
  • 主办单位:中国科学院微生物研究所 中国菌物学会
  • 主编:戴玉成
  • 地址:北京市朝阳区北辰西路1号院3号中国科学院微生物研究所B401
  • 邮编:100101
  • 邮箱:jwxt@im.ac.cn
  • 电话:010-64807521
  • 国际标准刊号:ISSN:1672-6472
  • 国内统一刊号:ISSN:11-5180/Q
  • 邮发代号:2-499
  • 获奖情况:
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,美国剑桥科学文摘,中国中国科技核心期刊,中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:5637