中文摘要：

目的探讨前列腺癌冷冻消融后对机体抗肿瘤免疫反应的影响。方法复制前列腺癌皮下移植瘤。将动物模型随机分为：对照组（A组）、手术切除组（B组）、冷冻消融组（C组）。分别于治疗前、治疗后7、14、21d取外周血、引流淋巴结、脾脏。ELISA法检测外周血干扰素（IFN），白细胞介素（IL）4浓度，以IFN-γ／IL-4计算Th1／Th2比值；分离引流淋巴结、脾脏淋巴细胞，ELISPOT试验检测CIM＋Th肿瘤特异性IFN-γ的分泌情况，LDH释放试验检测CD8＋CTL肿瘤杀伤活性。观察各组动物肿瘤转移情况。结果治疗后7d，A、B、C组外周血Th1／Th2比值分别为：4．97±0．31，10．07±0．62，13．71-t-O．57（P〈0．05）。引流淋巴结每10。个CIM＋111肿瘤特异性IFN-γ细胞数分别为22．3±1．0、24．0±1．2、243．4±46．2（P〈0．05），引流淋巴结CD8＋CTL特异杀伤活性分别为：（14．6±1．1）％、（15．2±0．8）％、（62．6±2．3）％（P〈0．05）。但各观察时间点A、B、C组脾脏11h细胞IFN-γ分泌及CTL细胞杀伤活性均无明显变化。术后28dA、B、C淋巴结转移率分别为100％、80％、40％，肺转移率均为100％。结论冷冻消融治疗前列腺癌可诱导机体Th1抗肿瘤免疫优势，并诱导引流淋巴结T细胞肿瘤特异性免疫反应，但存在一定局限性，尚需更深入研究。

英文摘要：

Objective To assess the anti-tumor immune response of cryoablation for prostate cancer. Methods Mouse model of prostate cancer was established. And the tumor-bearing mice were divided into three groups： control group （Group A）, surgery group （Group B） and cryoablation group （Group C） . Blood samples were withdrawn before and at Days 7, 14, 21 post-treatment. IFN-γand IL4 were analyzed by enzyme-linked immunosorbent assay （ELISA）. Thl/Th2 ratio was estimated from the IFN-γ/IL-d ratio. Lymphocytes of draining lymph node （DLN） and spleen were isolated. And the posttherapeutical number of tumor-specific IFN-γ CD4＋ Th cells was measured by the method of enzyme link immunal spot （ELISPOT）. And tumor-specific cytolytic activity of CD8 ＋ cytotoxic T lymphocyte （CTL） was measured by LDH assay. The rate of metastasis was assessed. Results At Day 7 post-treatment in Groups A, B and C, Thl/Th2 ratio was 4.97 ±0.31, 10.07±0.62 and 13.71±0.57 respectively （P〈 0. 05 ） ; the number of IFN-γ cells every 106 CD4 ＋ Th cells in DLN 22.3± 1.0, 24. 0± 1.2 and 243.4± 46.2 respectively ; cytolytic activity of cytotoxic T lymphocyte against prostate cancer cells （ 14. 6 ± 1.1 ） %, （15.2 ±0. 8）% and （62. 6±2. 3）% respectively（P 〈0. 05）. But for T cells derived from spleen, there were no difference in IFN-γ CD4 ＋ Th cells or cytolytic activity of CTL among the groups. At Day 28 posttreatment, the rate of DLN metastasis was 100% , 80% and 40% respectively. And the rates of lung metastasis were all 100%. Condusion Cryoablation for prostate cancer can induce the Thl advantage of anti-tumor immunity and generate the tumor-specific immune response in DLN. But the response remains limited. Further studies are warranted.