中文摘要：

目的探讨亚急性1-溴丙烷（1-BP）吸入染毒对雄性大鼠大脑海马区突触特异性标志物突触素（SYP）、谷氨酸受体2亚基（GluR2）、N-甲基-D-天冬氨酸受体2B亚基（NR2B）蛋白表达的影响。方法将48只无特定病原体级成年雄性Wistar大鼠按体质量随机分为对照组和低、中、高剂量组4组,每组12只。采用动式吸入染毒法,对照组给予新鲜空气,各剂量组分别予质量浓度为1 250、2 500和5 000 mg/m31-BP吸入,每天染毒6 h,每周染毒5 d,连续4周。染毒结束后处死大鼠,取出全脑,分离大脑（含海马组织）、脑干和小脑,分别采用实时荧光定量聚合酶链反应和免疫印迹法检测大鼠海马组织SYP、GluR2、NR2B的mRNA和蛋白相对表达水平。结果高剂量组大鼠于染毒第3周时出现反应迟钝和后肢肌力下降等表现。高剂量组大鼠染毒第1～4周体质量均低于同时间点对照组（P〈0.01）。高剂量组大鼠全脑、大脑和脑干质量均低于对照组（P〈0.05）。高剂量组大鼠海马组织海马3区和齿状回区可见少量神经元细胞坏死。各组大鼠海马组织SYP、GluR2和NR2B的mRNA相对表达水平分别比较,差异均无统计学意义（P〉0.05）。各组大鼠海马组织SYP蛋白相对表达水平比较差异无统计学意义（P〉0.05）;高剂量组大鼠海马组织GluR2蛋白相对表达水平低于对照组（P〈0.05）,NR2B蛋白相对表达水平高于对照组（P〈0.05）。结论大鼠海马组织GluR2和NR2B蛋白可作为1-BP中枢神经毒性的生物标志物,但其具体的生理意义尚需进一步研究。

英文摘要：

Objective To study the potential effects of subacute 1-bromopropane（ 1-BP） inhalation on the expression of synapse specific biomarkers synaptophysin（ SYP）,glutamate receptor 2（ GluR2） and N-methyl-D-aspartate receptor 2B（ NR2B） in the hippocampus of male rats. Methods Forty-eight specific pathogen free adult male Wistar rats were randomly divided into control group,low-,medium-,and high-dose groups according to body weight. Each group consisted of 12 rats. By dynamic inhalation intoxication method,the control group was exposed to fresh air,the dose groups were given 1 250,2 500 and 5 000 mg / m3 of 1-BP respectively,6 hours per day,5 days per week for continuous 4 weeks. After the exposure,the rats were executed and the whole brains were separated into cerebrum（ included hippocampus）,brainstem and cerebellum. Real time quantitative polymerase chain reaction and Western blot were used for detection of SYP,GluR2 and NR2 B mRNA and protein expression in hippocampus. Results Slow response and muscle strength descended in hind limbs were found in high-dose group in the 3rd week. Body weights of rats in high-dose group were lower than those of control group from the 1st to the 4th week（ P〈0. 01）. Weights of whole brain,cerebrum and brainstem in high-dose group were lower than those of control group（ P〈0. 05）. Rats in high-does group were found neuron necrosis in hippocampus cornu ammonis 3 and dentate gyrus region. No significant difference was found in SYP,GluR2 and NR2 B mRNA relative expression in all groups（ P〉0. 05）. No significant difference was found in SYP protein relative expression in different groups（ P〈0. 05）. The GluR2 protein relative expression in high-dose group was lower than that of control group（ P〈0. 05）. The NR2 B protein relative expression was higher than that of control group（ P〈0. 05）. Conclusion The GluR2 and NR2 B protein expression in hippocampus can be potential biomarkers for 1-BP central neurotoxicity,but its physiological meaning ne