中文摘要：

目的：前期研究发现叉头框（forkheadbox，Fox）转录因子Foxf2mRNA在乳腺淋巴结转移癌中的表达较对应的原发癌下调，是潜在的乳腺癌转移相关基因。本研究通过大样本临床病例检测（分析），阐明乳腺原发癌组织中Foxf2mRNA水平与临床病理因素的关系，评估其对预测患者预后的临床价值。方法：采用实时定量PCR方法检测233例乳腺原发癌组织中Foxf2mRNA表达水平；X2检验比较组间差异；Kaplan—Meier法绘制生存曲线；Log—rank时序检验比较组间生存期的差异。结果：Foxf2mRNA低表达率在乳腺癌临床Ⅰ～Ⅱ期低于Ⅲ期组（P=0．003），ER阳性组低于ER阴性组（P=0．014），随着阳性淋巴结数的增加，Foxf2mRNA低表达率逐渐升高（P=0．041），Foxf2mRNA低水平组患者5年无病生存率低于高水平组（P=0．001）。结论：Foxf2mRNA低表达与肿瘤进展和患者预后不良相关，Foxf2mRNA水平是潜在的乳腺癌患者预后分子标志物。

英文摘要：

Objective： To clarify the relationship between Foxf2 mRNA level and clinicopathologic factors in breast cancer, and to evaluate its clinical value in predicting the prognosis of breast cancer patients based on a large-sample data. Methods： Real-time reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the mRNA expression level of Foxf2 in primary breast carcinoma of 233 cases which were followed up for more than 3 years. Chi square test was used to compare the differences between/among groups. Survival analysis was carried out using Kaplan-Meier analysis. Log-rank test was used to compare the differences in survival. Results： The expression of Foxf2 mRNA in stage Ⅲ breast cancer was lower than that in stage Ⅰ-Ⅱbreast cancer （P=0.003）. The expression of Foxf2 mRNA in ER-positive breast cancer was lower than that in ER-negative breast cancer （P=0.014）. The expression of Foxf2 mRNA decreased as the number of positive lymph nodes increased （P=0.041）. Disease-free survival of patients with low Foxf2 mRNA expression was lower than those with high Foxf2 mRNA expression （P=0.008）. Conclusion： Low Foxf2 mRNA expression is associated with the development of breast cancer and poor prognosis and can be a potential molecular marker for predicting the prognosis of breast cancer patients.