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中文摘要：
从腐烂枯叶及附近土壤筛选到纤维素分解菌并研究其酶学特性。采用改进的赫奇逊分离法筛选纤维素分解菌。结果表明,共分离得到两株产纤维素酶的菌株。经菌落形态观察,初步鉴定一株为真菌（F-1）,另一株为细菌（B-1）。酶学性质初步研究显示,这两株菌的纤维素酶在酸性条件下具有较高的酶活（真菌F-1最适pH为5.5,细菌B-1最适pH为4.5）,酶最适反应温度分别为45℃和35℃,且真菌纤维素酶的耐热性较强;Ca2＋对酶反应有抑制作用。如该纤维素酶通过生物酶工程进行工业化生产改造,那么在环境净化方面,尤其是酸性环境下的废物处理中,将会具有很大的应用价值。
英文摘要：
Cellulose-decomposing strains were obtained from decayed leaves and neighboring soil, their enzymatic properties were further studied. Two cellulose-decomposing strains were isolated by the method of improved Hutchinson Medium. They were identified as fungus （F-1） and bacteria （B-I） based on colony morphology. The optimal pH value for the enzymatic reaction was 5.5 for F-1 and 4.5 for B-1. The suitable reaction temperature was 45 ℃ for F-land 35 ℃ for B-1. F-1 had higher heat tolerance than B-1. Ca2＋ was an inhibitor of the enzymatic reaction. If the cellulase improved by enzyme engineering technology,they have a great application value in the environmental purification, especially waste disposal in the acid condition.
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