该文以污染严重的珠江广州河段为研究对象,分析了感潮河段沉积物中的理化性质(机械组成、碳氮、溶解氧)和硝化细菌数量的垂向分布,并利用当地菌种,设计溶解氧浓度梯度培养硝化细菌,探讨溶解氧对硝化细菌的影响。结果表明,沉积物中的溶解氧在0~10cm间由0.26mg/L降低到0.02mg/L,这主要与粉砂为主的机械组成和底栖动物的活动有关;沉积物中的硝化细菌主要受溶解氧浓度的影响,室内培养与野外实验结果对照显示,沉积物中的硝化细菌在低溶解氧条件下处于抑制状态,并导致氨氮的积累;在溶解氧浓度低于0.03mg/L时氨氧化细菌的数量比亚硝酸氧化细菌高。
The aim of this study was to investigate the effect of dissolved oxygen (DO) on nitrifying bac- terial abundance and vertical distribution in sediment of Pearl River (Guangzhou section). Vertical dis- tributions of sediment physical-chemical properties ( grain size distribution, concentrations of total organic carbon, total nitrogen, nitrate, ammonium, DO) and nitrifying bacteria abundance were characterized u- sing sediment cores. The experiments were conducted to cultivate bacteria using local bacteria and differ- ent DO concentrations. The results showed that DO concentrations decreased from 0. 26 to 0. 02 mg· L-1 within sediment depths of 0 to 10 cm, which was attributable to the sandy sediment and perturbation of benthic macrofauna. The nitrifying bacteria were inhibited by low DO condition, resulting in ammoni- um accumulation in subsurface sediment. With DO 〈 0. 03 mg· L-1 , the abundance of ammonia oxidi- zing bacteria was greater than that of nitrite oxidizing bacteria.