中文摘要：

许多区分的植物机关有能力经由 de novo organogenesis 在分开以后再生进新植物。在从 Arabidopsis thaliana 叶将生物的活组织移植于培养基中培养的 de novo 根 organogenesis 期间，首先伤害在叶肉细胞导致内长的植物生长素生产。植物生长素当时是极的搬运了到，并且积累在，新生能干的房间接近创伤触发房间命运转变。家庭蛋白质催化的 ARABIDOPSIS (TAA ) 家庭蛋白质和 YUCCA (YUC ) 的色氨酸 AMINOTRANSFERASE 在植物生长素生物的续生说的二连续的生物化学的步，和 YUC 被显示了在 de novo 根 organogenesis 期间在叶肉房间涉及植物生长素生产。在这研究，我们证明 TAA 家庭也被要求为偶然 rooting。TAA 的抑制堵住了偶然从叶将生物的活组织移植于培养基中培养的根形成。有趣地，而 YUC1 和 YUC4 被显示了被伤害高度导致， TAA 基因在叶分开前后保留了一致表示层次。因此，我们建议 TAA 和 YUC 两个都在叶将生物的活组织移植于培养基中培养为植物生长素生物的续生说被要求，但是他们在新生起不同作用。当 YUC1 和 YUC4 响应伤害在植物生长素生合成催化限制率的步工作时， TAA 可能用作从叶将生物的活组织移植于培养基中培养在 de novo 根 organogenesis 期间忍受和基础酶。

英文摘要：

Many differentiated plant organs have the ability to regenerate into a new plant after detachment via de novo organogenesis. During de novo root organogenesis from Arabidopsis thaliana leaf explants, wounding first induces endogenous auxin production in mesophyll cells. Auxin is then polar transported to, and accumulates in, regeneration- competent cells near the wound to trigger the cell-fate transition. The TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS （TAA） family proteins and the YUCCA （YUC） family proteins catalyze two successive biochemical steps in auxin biogenesis, and YUCs have been shown to be involved in auxin production in meso- phyll cells during de novo root organogenesis. In this study, we show that the TAA family is also required for adventitious rooting. Inhibition of TAA blocked adventi- tious root formation from leaf explants. Intriguingly, whereas YUC1 and YUC4 have been shown to be highly induced by wounding, TAA genes retained consistent expression levels before and after leaf detachment. Therefore, we suggest that TAAs and YUCs are both required for auxin biogenesis in leaf explants, but they play different roles in regeneration. While YUC1 and YUC4 function in response to wounding to catalyze the rate- limiting step in auxin biosynthesis, TAAs probably serve as abiding and basal enzymes during de novo root organo- genesis from leaf explants.