中文摘要：

目的研究中国南海硬棘软珊瑚Scleronephthya sp.生物活性成分。方法利用正、反相硅胶柱层析、Sephadex LH-20以及HPLC等层析手段对一种硬棘软珊瑚中的生物活性成分进行分离。应用波谱分析技术并结合文献数据,对所得的化合物进行结构鉴定。采用琼脂扩散试验对分离得到的化合物进行体外抗微生物活性测试。结果和结论从该种硬棘软珊瑚中分离得到3个过氧化麦角甾醇（1～3）、鲛肝醇（4）、鲨肝醇（5）和1,2-O-十八烷基甘油缩醛（6）。初步体外抗微生物活性筛选发现这些化合物均有不同程度的抗菌活性。化合物5对大肠杆菌和巨大芽孢杆菌具有一定的抑制活性,化合物6对花药黑粉菌具有很强的抑制活性。化合物1～4仅显示微弱的抗微生物活性。

英文摘要：

Objective To investigate the bioactive constituents of coral Scleronephthya sp.collected from the South China Sea.Methods The compounds were isolated and purified using repeated column chromatographies on Sephadex LH-20,normal-and reversed-phase silica gels,and RP-HPLC.The structures of the compounds were elucidated based on the detailed spectroscopic analysis in combination with reported data.The in vitro antimicrobial activity of these compounds were assessed by an agar diffusion test.Results and conclusion Three 5α,8α-epidioxysterols,two alkyl glycerol ethers and 1,2-O-alkyl glycerol were isolated from Scleronephthya sp.;these compounds displayed different levels of antifungal and antibacterial activities in bioassay in vitro.Compound 5 inhibited the growth of Gram-negative bacterium Escherichia coli and the Gram-positive bacterium Bacillus megaterium.Compound 6 showed marked antifungal activity against Microbotryum violaceum.Compounds 1-4 demonstrated a weak antimicrobial activity.