中文摘要：

目的：考察不同采收期山绿茶药材中绿原酸和芦丁的含量及其变化规律,为探索山绿茶最佳采收期提供依据。方法：从2011年7月至2012年5月,每隔单月采摘药材1次,共6次。采用HPLC测定,Phenomenex Gemini C18色谱柱（4.6mm×250 mm,5μm）,以乙腈和0.1%磷酸进行梯度洗脱,检测波长330 nm,柱温30℃,流速1.0 mL·min-1。结果：绿原酸和芦丁分别在0.10-2.00μg,0.25~5.00μg峰面积与进样量呈良好的线性关系,回归方程分别为Y=4 027X＋5.262（r=1.000 0）,Y=1 258X-1.406（r=1.000 0）,平均回收率分别为97.18%和99.36%,RSD分别为1.27%和1.91%。1月份采收的山绿茶药材绿原酸和芦丁含量较高,7月份采收的山绿茶药材绿原酸和芦丁含量较低,山绿茶药材的合理采收时间可定为1月。结论：HPLC测定山绿茶中的绿原酸和芦丁含量的方法分离度良好,快速、准确、稳定、重复性好,可用于山绿茶药材及其炮制品的质量控制。山绿茶在生长发育过程中,绿原酸和芦丁含量会发生变化且有一定规律。

英文摘要：

Objective： Changing regularity of chlorogenic acid and rutin content were detected from Ilex hainanensis gathered in different periods by HPLC in order to decide the best gathering time.Method： Six samples of I.hainanensis were collected from July 2011 to May 2012 at every two months.RP-HPLC was used to determine the content of chlorogenie acid and rutin in different periods of I.hainanensis with the chromatographic conditions of Phenomenex Gemini C 18 column（4.6 mm × 250 mm,5 μm）,detected at the wavelength of 330 nm,gradient elution of acetonitrile-0.1% phosphoric acid solution as the mobile phase,column temperature of 30 ℃,the flow rate was 1 mL·min-1.Result： The linearity of chlorogenic acid and Rutin were good in the range of 0.10-2.00 μg and 0.25-5.00 μg,regress equation were Y = 4 027 X ＋ 5.262（r = 1.000 0） and Y = 1 258 X-1.406（r = 1.000 0）,the average recovery was 97.18% and 99.36%,RSDs were 1.27%,1.91%.The content of Chlorogenic acid and Rutin were higher in I.hainanensis collected in January,and the content of Chlorogenie acid and Rutin were lower in I.hainanensis collected in July.So the collection time could be set at January.Conclusion： The determination method of chlorogenic acid and rutin in I.hainanensis is good at resolution and reproducibility,quickly analysis,accurate and stable,which can be used for the quality control of I.hainanensis.There are some changing regulations of chlorogenic acid and rutin in llex hainanensis during its growing development period.The content of chlorogenic acid and rutin will help us to decide the best gathering time for I.hainanensis.