中文摘要：

目的建立同时测定人参及其制剂中16种人参皂苷的HPLC方法。方法采用C18（150mm×4．6mm，5μm）色谱柱；流动相为乙腈和水，梯度洗脱，体积流量1．0mL／min，检测波长203nm，柱温35℃。结果16种人参皂苷Rgl、Re、Rf、Rbl、Rg2、Rc、Rb2、Rb3、F1、Rd、F2、Rg3、Rh2及原人参三醇、compoundK、原人参二醇均得到良好分离，线性关系良好（r≥0．9990）。加样回收率均在95％-102％，RSD〈2％。结论该方法快捷简便、稳定可靠，可应用于人参及其制剂的质量控制。

英文摘要：

Objective In order to evaluate the quality of Panax ginseng and its preparation, a simple and accurate HPLC method for determining the contents of 16 ginsenosides from P ginseng was established. Methods The chromatographic separation was achieved on a C18 column （150 mm ×4.6 mm, 5μm） using a mobile phase made up of acetonitrie and water at a flow rate of 1.0 mL/min. The detection wavelength and column temperature were set as 203 nm and 35 ℃, respectively. Results Sixteen ginsenosides （Rgl, Re, Rf, Rbl, Rg2, Rc, Rb2, Rb3, F1, Rd, F2, Rg3, protopanaxatriol, compound K, Rh2, and protopanaxadiol） were separated at baseline with good linearity （r 〉 0.999 0）. The recovery rates were 95%-102% （RSD 〈 2%）. Conclusion The method is simple, fast, accurate, and could be applied to the quality control of P. ginseng and its preparation.