中文摘要：

目的：对水稻线粒体基因orf288功能进行初步探索。方法：笔者在大肠杆菌中构建水稻线粒体基因orf288的原核表达载体pET-30a—ory288，转化入BL21（DE3）后利用IPTG诱导，绘制生长曲线，分析其表达产物对细菌生命活动的影响。结果：成功构建了pET-30a—orf288重组子，并能成功表达ORF288。结论：原核表达产物ORF288对细菌的生长具有明显的抑制作用，为进一步研究orf288作用提供了研究线索。

英文摘要：

Objective： The function of rice mitochondrial gene orf288 in a preliminary exploration. Method： To study the molecular mecha-nism of this gene,the sequence of orf288 gene was cloned into pET- 30a, and then expressed in BL21 （DE3） strain. Result： The results showed that weakly expressed native ORF288 proteins induced from the recombinant pET - 30a - orf288 strongly inhibit the growth of E-.coli. Conclusion： These results may provide a clue to get some information about the mechanism of orf288.