中文摘要：

目的探讨参芪扶正注射液（SQFZ）对胰腺癌多药耐药的逆转作用及初步分子机制。方法以胰腺癌细胞株Patu8988为研究对象，采用氟尿嘧啶（5-fu）诱导得到Patu8988／5-Fu耐药细胞株；采用四甲偶氮唑蓝（MTT）法测定Patu8988／5-Fu耐药倍数、SQFZ的细胞增殖抑制作用以及SQFZ对Pmu8988／5-Fu耐药细胞株的逆转倍数；采用流式细胞分析技术（FCM）测定SQFZ对细胞内罗丹明123（Rh0123）蓄积的影响；采用实时定量PCR技术检测SQFZ对耐药细胞株MDR1、Bcl-2和Bax基因表达的调控作用；采用蛋白质免疫印迹（Western Blot）技术检测SQFZ对耐药细胞株Bcl-2、Bax和P-gP蛋白表达情况影响。结果浓度为5、10和20μL·mL^-1的SQFZ可逆转Patu8988／5-Fu细胞的耐药性，逆转倍数依次为1．5、2．3和3．4倍。SQFZ剂量依赖性地增加Patu8988／5-Fu细胞的Rho123蓄积量（r=0．979，P〈0．05）。分子机制研究表明，SQFZ下调Patu8988／5-Fu细胞MDR1和Bcl-的基因表达，上调Bax基因表达。SQFZ可使Pmu8988／5-Fu细胞中P-gP和Bcl-2的蛋白表达水平下降，使Bax蛋白表达水平上升。结论SQFZ具有逆转Patu8988／5-Fu细胞多药耐药的作用，其作用机制可能与MDRI、Bcl-2和Bax基因的转录及蛋白表达有关。

英文摘要：

OBJECTIVE To explore the reversal effect and molecular mechanism of shengqi fuzheng injection （SQFZ） on pancreatic cancer muhidrug resistance（MDR） ceils. METHODS Patu8988 was exposured to fluorouracil（5-Fu） to obtain stable 5-Fu-resistant Patu8988/5-Fu cell lines. MTT assay was used to detect the inhibition of SQFZ on cell proliferation and the resistance index of Patu8988/5-Fu and reversal index of SQFZ were calculated. Flow cytometry （FCM） was employed to measure the accumulation of Rho123 in cells. The mRNA expression of MDR1, Bcl-2 and Bax and presence of Bcl-2, Bax and P-gp protein were analyzed by quantitative real-time PCR and Western blot methods respectively. RESULTS SQFZ at the concentration of 5,10,20 μL·mL^-1 obviously reversed MDR of Patu8988/5-Fu cells, with the reversal index of 1.5,2. 3 and 3.4 respectively. It increased Rho123 accumulation in a dose-dependent manner in MDR cells（r = 0. 979, P 〈 0. 05）. The study revealed that SQFZ downregulated the expressions of MDR1 and Bcl2 gene and upregulated the expression of Bax gene. Furthermore, SQFZ decreased the expression of P-gp and Bcl-2 protein, and increased the expression of Bax protein in Patu8988/5-Fu cells. CONCLUSION SQFZ can reverse multidrug resistance of Patu8988/FU cells, likely by modulating gene transcription and protein expression of MDR1, Bcl-2 and Bax.