欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的：探讨百草枯（PQ）对活性氧类物质（ROS）和中性粒细胞凋亡的影响及可能机制。方法：离体培养健康成人中性粒细胞,给予不同浓度百草枯刺激后培养6~24 h,流式细胞仪检测中性粒细胞凋亡率及ROS含量,Western blot检测信号蛋白核因子kappa B（NF-кB）、天冬氨酸特异性半胱氨酸蛋白酶3（Caspase 3）;给予ROS、NF-κB拮抗剂后,再次检测中性粒细胞凋亡率及信号蛋白含量。结果：PQ可明显促进ROS产生及抑制中性粒细胞凋亡,这种效应可被ROS抑制剂二联苯碘（DPI）及NF-кB抑制剂四氢吡咯二硫代氨基甲酯（PDTC）逆转。同时,PQ能促进NF-κB表达增加,而Caspase 3表达则受到明显抑制。结论：百草枯是强力的ROS诱导剂,并能抑制中性粒细胞凋亡,其机制可能与NF-κB活化,进而抑制Caspase 3表达有关。
英文摘要：
Objective： To investigate the effect of paraquat（PQ） on reactive oxygen species（ROS） and neutrophil apoptosis and its possi- ble signal transductian pathways. Methods： Cultm＇ed neutrophils were treated with different concentrations of PQ for 6 ~ 24 h. The apoptosis rate of neutrophils and ROS content were determined by flow cytometry. The exoressions of nuclear factor kappa B （NF-KB） and Caspase 3 were detected by Western blot. These parameters were checked again after NF-KB and Caspase 3 antagonist were applied. Results： PQ could boost ROS generation and depress neutrophil apoptosis significantly. At the same time PQ could enhance the expression of NF-KB and inhibit the expression of Caspase 3. These effects could be reversed by ROS inhibitor diphenyleneiodonium （DPI） and NF-KB inhibitor pyrro- lidinedithiocarbamate（PDTC）. Conclusion： PQ is a potent inducer of ROS and can inhibit neutrophil apoptosis by activating NF-KB and sur- pressing Caspase 3 activity.
同期刊论文项目
同项目期刊论文
期刊信息
位置：