中文摘要：

H9N2亚型禽流感病毒的血凝素（HA）基因经树突状细胞靶向肽（DCpep）与异亮氨酸拉链（GCN4）基因修饰后，插入到穿梭表达栽体pSIP409中，将质粒电转化至乳酸杆菌内。结果表明，该重组乳酸杆菌经诱导后，能够表达大小约为63000且具有反应原性的蛋白，为进一步探讨重组乳酸杆菌在激发黏膜免疫反应过程中，DC调控黏膜免疫应答的机制及研制抗禽流感口服疫苗奠定基础。

英文摘要：

After modified by DCpep and GCN4 genes,the hemagglutinin （HA） gene of HgN2 sub- type avian influenza virus was inserted into the shuttle vector pSIP409. The recombinant plasmid was electric-transformed into Lactobacillus. After induction,a protein about 63 000 was detected by Western blot, which indicated its reactionogenicity. It provides the basis for further studying the mechanism of dendritic cells in the regulation of mucosal immune responses and the development of oral vaccine against avian influenza.