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中文摘要：
目的检测HMGB1和髓系来源的免疫抑制性细胞（MDSC）在宫颈癌肿瘤微环境中的表达水平,分析它们在宫颈癌发生、发展中的作用及相互关系。方法用人宫颈癌Hela细胞系建立裸鼠皮下瘤模型,成瘤后取荷瘤小鼠的脾脏、肿瘤组织及外周血制成单细胞悬液,通过流式细胞术检测脾脏、外周血中MDSC细胞的百分比。分别采用Western blot、RT-q PCR方法检测肿瘤组织中HMGB1蛋白和m RNA的表达水平。结果与正常对照组相比,荷瘤鼠外周血和脾脏中CD11b＋Gr-1＋MDSC的比例明显增高（P〈0.05）。与癌旁组织对照组相比,荷瘤鼠肿瘤组织中HMGB1呈明显高表达,具有统计学意义（P〈0.05）。荷瘤鼠脾脏中CD11b＋Gr-1＋MDSC增高与肿瘤组织中HMGB1的高表达呈正相关。结论宫颈癌肿瘤微环境中HMGB1可能通过调控MDSC参与宫颈癌的发生、发展。
英文摘要：
This study designed to detect the levels of HMGB1 and MDSC in tumor microenvironment of cervical cancer and explore their clinical significances and correlations. Cervical cancer cells Hela were used to construct nude mice subcutaneous tumor models. When tumor formed, the peripheral blood and spleen were isolated and the percentage of CDllb＋Gr-I~MDSC was detected by FCM. The mRNA and protein expression of HMGB1 were measured by real-time quantitative （RT-qPCR） and Western blotting respectively in tumor tissues and tumor- adjacent normal tissues from tumor-bearing mice. Compared with the normal mice, the percentage of CD11b＋Gr-1＋ MDSC in peripheral blood and spleen of tumor-bearing mice were obviously increased （P〈 0.05）; the expression of HMGB1 in tumor tissues was significantly higher than that in tumor-adjacent normal tissues （P〈 0.05）. Furthermore, HMGB1 had a positive correlation with MDSC in tumor-bearing mice. Taken together, in cervical tumor microenvironment, HMGBI may participate in the occurrence and development of cervical cancer through regulating MDSC.
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