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鲍鱼脏器多糖的抗氧化活性研究
  • ISSN号:1003-5788
  • 期刊名称:《食品与机械》
  • 时间:0
  • 分类:TS218[轻工技术与工程—粮食、油脂及植物蛋白工程;轻工技术与工程—食品科学与工程] TS202.3[轻工技术与工程—食品科学;轻工技术与工程—食品科学与工程]
  • 作者机构:[1]大连工业大学,辽宁大连116034
  • 相关基金:国家科技部国际重大合作项目(项目编号:2006DFA32580)
中文摘要:

采用酶法分别从雌、雄性皱纹盘鲍脏器中提取鲍鱼脏器粗多糖(AVP)。经Sephadex G-100凝胶过滤柱分离后,FAVP和MAVP各得到两个组分:FAVPⅠ,FAVPⅡ;MAVPⅠ,MAVPⅡ。它们清除羟自由基的EC50分别为1.38,0,99,1.51,1.19mg/mL,与对照Vc(EC50为1.23mg/mL)相比活性更强;其还原能力的A700,0.2分别为37.68,7.37,22.41,7.63mg/mL,与对照Vc(EC20为30.57μg/mL)相比弱很多;其络合能力则很弱。相关分析结果表明,鲍鱼脏器多糖具有一定的抗氧化能力。

英文摘要:

The crude polysaccharides termed as AVP was extracted from female and male abalone viscera (Haliotis discus hannai Ino) by protease extraction. The female and male AVP (FAVE and MAVP) were fractionated by gel filtration chromatography with scphadex G -100, each giving two fractions containing polysaccharide conjugates termed FAVP Ⅰ , FAVP Ⅱ , MAVP Ⅰ , MAVP Ⅱ. The EC50 values of scavenging activity on hydroxyl radical of the four fractions were 1. 38, 0. 99, 1. 51, 1.19 mg/mL respectively, which were higher than that of positive control Vc(EC50: 1.23 mg/mL). The A700, 0.2 values of reducing power were 37.68, 7.37, 22.41,7.63 mg/mL respectively, which were lower than that of positive control Vc ( A700, 0.2 : 30.57 μg/mL). The chelating ability of the fractions was undetectable. Based on the correlation analysis, there were negative correlation between scavenging activity on hydroxyl radical and polysaccharide content. However, the exact relationship between the polysaccharide and protein content and antioxidant need further studies.

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期刊信息
  • 《食品与机械》
  • 北大核心期刊(2011版)
  • 主管单位:湖南省教育厅
  • 主办单位:长沙理工大学
  • 主编:黄寿恩
  • 地址:长沙市万家丽南路二段960号
  • 邮编:410004
  • 邮箱:foodmm@21cn.com
  • 电话:0731-85258200 85258201
  • 国际标准刊号:ISSN:1003-5788
  • 国内统一刊号:ISSN:43-1183/TS
  • 邮发代号:42-83
  • 获奖情况:
  • 一级期刊
  • 国内外数据库收录:
  • 中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:20609