研究了反库斯塔克亚种(kurstaki)毒素(65kDa)在高岭土、针铁矿和氧化硅表面的吸附和解吸特性.结果表明:在磷酸盐缓冲体系(pH8)中,3种矿物的等温吸附曲线均符合Langmuir方程(R^2〉0.9661),它们对反毒素的吸附顺序为:针铁矿〉高岭土〉二氧化硅.矿物对Bt毒素的吸附1h就基本达到了吸附平衡.在pH6~8范围内,针铁矿、高岭土和二氧化硅对Bt毒素的吸附量随pH值的升高而降低.10℃~50℃范围内,针铁矿和氧化硅对反毒素吸附量随温度升高有所下降(8.39%和47.06%),高岭土对反毒素吸附则略有升高(5.91%).红外光谱分析显示,Bt毒素被矿物吸附后结构仅有微小变化.被矿物吸附的Bt毒素不易被去离子水解吸,水洗3次总解吸率为28.48%~42.04%.
This paper studied the characteristics of the adsorption and desorption of Bt (Bacillus thuringiensis) toxin on goethite, kaolinite, and silica. The results showed that in phosphate buffer (pH 8 ), the adsorption isotherms of Bt toxin on the test minerals followed Langmuir equation (R2 〉0. 9661 ), and the adsorbed amounts were in the order of goethite 〉 kaolinite 〉 silica. The Bt toxin was easily adsorbed on the minerals, and the adsorption could reach equilibrium after 1 hour. Within the range of pH 6-8, the amounts of Bt toxin adsorbed on goethite, kaolinite and silica decreased with increasing pH ; in the range of 10 ℃-50 ℃, the amounts of the toxin adsorbed on goethite and silica decreased by 8.39% and 47.06%, respectively, while that on kaolinite increased slightly (5.91% ). The infrared absorption spectrum showed that there was only a minor alteration of Bt toxin after adsorption. The toxin adsorbed on the minerals was not easily desorbed by deionised water, with the desorption rate ranged from 28.48% to 42. 04% after three times washing.