中文摘要：

目的 探讨四氯化碳（CCl4）诱导大鼠肝纤维化脂质过氧化相关蛋白表达的动态变化及一贯煎的干预效应.方法 Wistar雄性大鼠57只,其中模型组39只,正常组18只.模型大鼠腹腔注射50％的CCl4橄榄油溶液（1ml/lg）,每周2次,共9周.造模3、6周后,随机抽取正常及模型大鼠各6只,处死作动态观察.其余模型大鼠随机分为模型组15只及干预组12只,模型组大鼠在8周时处死4只观察成模情况.第7周开始,继续造模的同时,干预组用一贯煎（2.682 g/kg）蒸馏水稀释灌胃,1次/d,共计3周.用药3周结束后,处死大鼠,检测肝功能、肝组织羟脯氨酸（Hyp）和丙二醛（MDA）含量、超氧化物歧化酶（SOD）与谷胱甘肽（GSH）活性,以及热休克蛋白70 （HSP70）、血红素加氧酶-1（HO-1）、转铁蛋白（Transferrin）、过氧化还原酶（Prxd）6、肝脏型脂肪酸结合蛋白（L-FABP）等的表达.计量资料采用单因素方差分析,计数资料采用Ridit分析. 结果 （1）与对照组比较,模型组大鼠6、9周时肝组织MDA含量显著升高[（4.23±0.45） nmol/mg比（2.22±0.59）nmol/mg; （6.29±1.23） nmol/mg比（2.22±0.59） nmol/mg,F值分别为60.13、66.99,P值均＜ 0.05]；SOD活性显著降低[（196.94±39.20） U/mg比（264.50±30.44）U/mg,F=11.12,P＜ 0.05; （152.21±51.65） U/mg比（264.50±30.44） U/mg,F=23.11,P＜0.01]；GSH含量显著降低[（48.47±7.27） nmol/mg比（60.74±9.04） nmol/mg,F=6.71,P＜0.05;（37.89±9.01） nmol/mg比（60.74±9.04）nmol/mg,F=24.06,P＜0.01]；与9周模型组比较,干预组MDA显著降低[（4.25±0.86） nmol/mg比（6.29±1.23） nmol/mg,F=19.52,P＜ 0.01],SOD显著升高[（198.35±46.48） U/mg比（152.21±51.65） U/mg,F=4.65,P＜0.05],GSH显著升高[（53.73±7.54） nmol/mg比（37.89±9.01） nmol/mg,F=19.23,P＜0.01]；（2）与正常组比较,9周时模型组大鼠HSP70蛋白表达量升高（1.21±0.06比0.58±0.07,F=166.87,P＜0.0l）,HO-1蛋白表达量也升高（1.11

英文摘要：

Objective To investigate the dynamic change of lipid peroxidation-related protein expression and the intervention effects of Yiguanjian （YGJ） Decoction on liver fibrosis induced by CCl4 in rat -.Methods Fifty-seven male Wistar rats were randomly divided into a liver fibrosis group （n =39） and a normal group （n =18）.The liver fibrosis was treated with peritoneal injection of 50% CCl4 for nine weeks.At the end of weeks 3 and 6 of CCl4 treatment,six rats were sacrificed to assess the status of- liver fibrosis.At the end of week 7,the remaining -fibrotic rats were randomly divided into an untreated model group （M,n =15） and a YGJ-treated group （n =12）.The YGJ group was administered daily,oral YGJ Decoction for three weeks,concomitant with continued CCl4 treatment.The M group and normal group received the same treatment oral regimen and volume of distilled water.At the end of week 8,four rats in group M were sacrificed to observed the fibrosis status.At the end of week 9,the fibrotic rats were sacrificed for sampling.Liver function,histological changes,contents of hydroxyproline （Hyp） and malondialdehyde （MDA）,activity of super oxidase dismutase （SOD） and L-glutathione （GSH）,protein expression of heat shock protein （HSP）70,heme oxygenase （HO）-1,transferrin,peroxiredoxin （Prxd） 6 and liver fatty acid binding protein （L-FABP）were detected.Results Compared with normal group-,the MDA content was increased significantly in M group - at week 6 （M：4.23 ± 0.45 nmol/mg vs.normal：2.22 ± 0.59 nmol/mg,F =60.13,P 〈 0.01） and week 9 （M：6.29 ± 1.23 nmol/mg vs.normal：2.22 ± 0.59 nmol/mg,F =66.99,P 〈 0.01）,but the SOD activity was decreased significantly at the same time points [week 6：（M：196.94 ± 39.20 U/mg vs.normal：264.50 ± 30.44U/mg,F =11.12,P 〈 0.01]）; [week 9：（M：152.2 ± 51.65 U/mg vs.normal：264.50 ± 30.44 U/mg,F =23.11,P 〈 0.01）],as were the GSH content [week 6：（M：48.47 ± 7.27 nmol/mg vs.60.74 ± 9.04 nmol/mg,F =6.71,P