中文摘要：

为了优化体细胞克隆胚胎体外培养液，提高体细胞的克隆效率，以CRlaa＋5％FBS＋0．3％BSA为基础培养液，分别添加0、60、80、100和120μmol／LH2O2观察延边黄牛体细胞克隆胚胎的氧化损伤情况和体外发育模式，并研究1、4和7mmol／LGSH对延边黄牛重组胚的保护作用。结果表明：（1）添加0、60、80μmol／LH2O2组卵裂率显著要高于100μmol／L和120μmol／L组（P〈0．05），添加H202的组囊胚发育率显著低于未添加组（P〈0．05）；（2）随着H2O2处理浓度的升高，重组胚发育速度增加，但是发育停滞现象也更明显；（3）在培养液中添加GSH后，1mmol／L组卵裂率显著高于7mmol／L组（P〈0．05），与0和4mmol／L组差异不显著（P〉0．05），囊胚发育率显著高于其它组（P〈0．05）。可见，在体细胞克隆胚胎体外发育过程中H2O2对其具有氧化损伤作用，不利其体外发育，在培养液中添加1mmol／LGSH对体细胞克隆胚胎的氧化损伤具有明显的保护作用。

英文摘要：

In order to optimize the culture solution and improve the efficiency of somatic cell nuclear transfer, oxidative damage and developmental mode in vitro of reconstructed embryos from somatic cell nuclear transfer in Yanbian cattle were observed in the experiment treated with addition of H2O2 to the base culture solution （CR1aa ＋ 5%FBS ＋ 0.3% BSA）,and the protective effect of GSH on reconstructed embryos was studied. The results showed that ： （ 1 ） The cleavage rate of the group with addition of 0, 60 and 80 μmol/L of H2O2 was significantly higher than that of 100 and 120 μmol/L （P 〈0.05 ）. The blastocyst rate of the group treated with H2 02 was significantly lower than that of the group without the treatment of H2 O2 （ P 〈 0.05 ） ; （ 2 ） The rate of development of reconstructed embryos increased along with the rise of the concentration of H2 O2, but the arrest phenomenon of development was much more obvious ; （ 3 ） The cleavage rate of the group withaddition of 1 mmol/L of GSH was significantly higher than that of the group treated with 7 mmol/L （P 〈 0.05 ）, but was not significantly hither than that of the group treated with 0 and 4 mmoL/L （ P 〉 0.05 ）. The blastocyst rate of the group treated with 1 mmol/L was strikingly higher than that of the other treatment groups （P 〈0.05）. It is thus evident that oxidative damage by H2O2 exists in the development of reconstructed embryos from somatic cell nuclear transfer, and it is harmful for the development of reconstructed embryos in vitro. An addition of 1 mmol/L of GSH in culture solution possesses obvious protective effect on oxidative damage to reconstructed embryos from somatic cell nuclear transfer.