中文摘要：

目的：基于CdTe量子点的荧光淬灭现象，建立一种简便、经济和高灵敏度的检测硫酸庆大霉素的新方法。方法：以巯基乙酸（TGA）为稳定剂，在水相中合成CdTe量子点，并用荧光分光光度计、红外光谱仪、透射电镜对其进行表征。实验过程中，优化了CdTe量子点TGA、镉、碲三者之间的比例，考察了不同pH与回流时间对量子点荧光性质的影响。通过测定CdTe量子点荧光强度的下降，实现了对硫酸庆大霉素注射液浓度的测定。结果：合成了荧光发射峰在607nm、粒径在4．0nm左右的CdTe量子点。硫酸庆大霉素对CdTe量子点的荧光有淬灭作用，在pH=7．4的磷酸盐缓冲体系中，硫酸庆大霉素的浓度在0．10～2．00mg／L范围时，量子点荧光强度比值与硫酸庆大霉素浓度符合Stern—Volmer方程且相关系数R^2=0．9989。结论：该方法灵敏度较高，快速简便，可用于硫酸庆大霉素浓度的测定。

英文摘要：

Objective： To prepare a facile and cost efficient method for the determination of gentamicin sulfate. Methods： The thiogycolic acid capped CdTe quantum dots were synthesized in aqueous phase. Transmission electron microscopy, infrared spectroscopy and fluorescence spectroscopy were used to characterize the particle size and shape, the surface chemical group and the fluorescence property of the quantum dots. During the experiment, proportion of TGA, Cd, Te among CdTe quantum synthesis process were optimized, and the optical stability and the effect of pH on the fluorescence intensity of CdTe quantum dots were also evaluated. Results： The fluorescence emission peak at 607 nm and the TEM images displayed CdTe at about 4.0 nm were synthesized. The fluorescence quenching effect of gentamicin sulfate on CdTe quantum dots was researched, and a new method for the detection of gentamicin sulfate using CdTe quantum dots as fluorescent probes was established. In the optimal conditions, CdTe quantum dots were successfully applied to the detection of gentamicin sulfate in water, and a linear relationship was obtained to cover the concentration range of 0.10-2.00 rag/L, with a correlation coefficient of 0.998 9, which was best described by a Stern-Volmer equation. Conclusion： The proposed method can be used for the determination of gentamicin sulfate with satisfactory results.