中文摘要：

本试验采用流式细胞仪对苜蓿（Medicago sativa L.）F1代杂种花药培养再生植株进行染色体倍性检测。旨在建立高效的苜蓿花药培养体系,提高苜蓿单倍体的诱导效率,寻求一种快速简便的苜蓿倍性检测方法。检测结果为流式细胞仪矩形图上清晰地检测出二倍体、三倍体、四倍体以及二倍体加四倍体的嵌合体。50株苜蓿花药再生植株中有42株四倍体（2n=4x）,2n=4x与2n=2x的混合体为5株,二倍体（2n=2x）为2株,三倍体（2n=3x）为1株。本文还讨论了苜蓿花药培养体系的建立,优化,以及筛选出了适宜制作苜蓿细胞悬浮液的缓冲液（15 mmol·L^-1Tris-HCl（pH7.5）;80 mmol·L^-1KCl;20 mmol·L^-1NaCl;2 mmol·L^-1EDTA-Na2;15 mmol·L^-1β-Mercaptoethanol;0.1%（V/V）TritonX-100;0.5 mmol·L^-1Spermine.4HCl）及其流式细胞仪检测苜蓿倍性的适应性程序。

英文摘要：

Ploidy levels of F1 hybrid plants,derived from anther culture in Medicago sativa L.,were determined by flow-cytometric analysis.Purpose of the study was to seek a fast and reliable method for DNA ploidy detection to establish an effective system of anther culture while improving haploid induction in Medicago sativa L.There were 42 tetraploids（2n=4x=32）,5 mixoploids of diploid and tetraploid（2n=4x ＋2n=2x）,2 diploids,1 triploid in 50 regeneration plants of anther culture.The optimization of both cell suspension buffer and anther culture system in Medicago sativa L.was also discussed in the paper.