中文摘要：

目的研究色素上皮衍生因子（pigment epithelium-derived factor,PEDF）对氧化低密度脂蛋白（oxidized low-density lipoprotein,Ox-LDL）所致血管内皮细胞损伤的抑制作用。方法体外培养人脐静脉内皮细胞（human umbilical vein endothelial cells,HUVECs）,建立Ox-LDL诱导的HUVECs损伤模型。Ox-LDL诱导损伤前,给予400μg/L PEDF预处理24 h,分别用MTT法和SYTO-13/PI双染法检测细胞活力和凋亡情况,Western blot技术检测β-连环蛋白（β-catenin）和蓬乱蛋白（disheveled-1,Dvl-1）表达水平。结果与对照组相比,Ox-LDL浓度为25 mg/L时细胞活力开始明显下降（P〈0.05）,且浓度达100 mg/L时,细胞存活率下降至最低为49.0%（P〈0.05）,成功建立Ox-LDL诱导的HUVECs损伤模型。在模型的基础上进行PEDF干预,细胞存活率提高约17%（P〈0.05）,细胞凋亡率降低约19%（P〈0.05）,β-catenin和Dvl-1表达显著下调（分别为10%、15%,P〈0.05）。结论 PEDF能够减轻Ox-LDL诱导的HUVECs损伤,且在减轻损伤的同时下调Ox-LDL诱导的β-catenin和DVL-1蛋白表达,这可能与PEDF抑制Wnt/β-catenin信号通路有关。

英文摘要：

Objective To investigate the inhibitory effect of pigment epithelium-derived factor（PEDF） on oxidized low density lipoprotein（Ox-LDL）-induced endothelial injury.Methods A cell injury model of human umbilical vein endothelial cells（HUVECs） was induced by Ox-LDL and intervened with PEDF.Cell viability of HUVECs was evaluated with MTT assay.Cell apoptosis of HUVECs was detected with MTT assay and SYTO-13/PI double staining.The expression of β-catenin（non-phosphorylated-β-catenin） and disheveled-1（Dvl-1） were analyzed by western blot.Results The Ox-LDL-induced HUVECs cell injury model was successfully established.Exposure of HUVECs to Ox-LDL led to a decrease in cell viability（P〈0.05）.Ox-LDL-induced HUVEC injury and apoptosis, the up-regulation of β-catenin and Dvl-1 expression were suppressed by PEDF pretreatment（P〈0.05,respectively）.Conclusion PEDF can ameliorate Ox-LDL-induced endothelial injury by down-regulating the expression of β-catenin and Dvl-1, which may be mediated by Wnt/β-catenin pathway.