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中国新疆野苹果[Malus sieversii(Lebed.)Roem.]群体遗传结构的SSR分析
  • ISSN号:1673-8527
  • 期刊名称:《遗传学报:英文版》
  • 时间:0
  • 分类:S435.111.4[农业科学—农业昆虫与害虫防治;农业科学—植物保护]
  • 作者机构:[1]山东农业大学作物生物学国家重点实验室,泰安271018
  • 相关基金:This work was supported by the National Natural Science Foundation of China (No. 30471196) and the Special Program for Doctorial Site of Universities (No. 200404344011).
中文摘要:

以中国新疆伊犁地区的巩留县莫合镇库尔德宁、新源县交吾托海、霍城县大西沟和塔城地区的裕民县巴尔鲁克山4个种下居群的109个新疆野苹果实生株系为材料,利用8对苹果SSR引物进行群体遗传结构的研究。结果表明:8对SSR引物在4个居群中可平均扩增出16条带,其中巩留县居群多态性带数百分比最高为89.06%,各位点平均Nei基因多样度为0.257.4个群体共扩增出128个位点,在种级水平及巩留县、新源县、霍城县和裕民县4个居群水平多态性位点百分比分别为100%、88.28%、84.38%、87.50%、78.12%,种级水平Nei基因多样度(H=0.2619)和香农信息指数(l=0.4082)大于种下居群,4个种下居群Nei基因多样度和香农信息指数比较巩留县〉霍城县〉新源县〉裕民县;巩留县居群和新源县居群遗传一致度最大,遗传距离最近;根据基因分化系数(GST=0.064)值,测得的基因流Nm为7.265。UPGMA聚类分析结果表明,巩留县和新源县居群遗传关系最近,霍城县居群次之,裕民县居群远离其他3个居群,巩留县、新源县、霍城县和裕民县4个居群是相对独立的群体,但同时存在部分基因交流。所有参数分析表明,巩留县遗传多样性最丰富,故在制定原位种质保护计划时应优先考虑巩留县居群。

英文摘要:

One hundred and nine Malus sieversii accessions from four geographical populations located at Kuerdening in Mohe town, Gongliu County, Jiaowutuohai, in Xinyuan County, Daxigou in Houcheng County of Ily State, and Baerluke Mountain in Yumin County of Tacheng State, Xinjiang Uygur Autonomous Region of China were studied by SSR markers. The purpose of the study was to determine the genetic structure and diversity in these eco-geographical populations with eight pair SSR primers of apple. The results indicated that: an average of 16 bands was detected in the four populations. The percentage of polymorphic bands in Gongliu population (89.06%) was the highest in the four populations. The average Nei's gene diversity index was 0.257 for all the loci. Totally, 128 polymorphic loci were detected and the percentage of polymorphic loci (P) was 100%, 88.28%, 84.83%, 87.50%, and 87.12%, respectively, at the species level and Gongliu, Xinyuan, Huocheng, and Yumin population levels. The Nei's gene diversity index (H = 0.2619) and Shannon's information index (1 = 0.4082) in the species level were higher than in the population level. The Nei's gene diversity index and Shannon's information index in the four populations were Gongliu 〉 Huocheng 〉 Xinyuan 〉 Yumin. Gongliu population and Xinyuan population were the highest in genetic identity and the closest in genetic distance. Gene flow between the populations was 7.265 based on genetic differentiation coefficient (GST = 0.064). The UPGMA cluster analysis indicated that the genetic relationships between the Gongliu and Xinyuan population were the closest, and the Yumin population were the farthest with the other three populations. The UPGMA cluster analysis indicated that the four geographical populations located in Gongliu, Xinyuan, Huocheng, and Yumin were relatively independent populations. Concurrently, there was also mild gene exchange between the populations. On the basis of the study of population genetic structure and the highest geneti

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期刊信息
  • 《遗传学报:英文版》
  • 北大核心期刊(2004版)
  • 主管单位:中国科学院
  • 主办单位:中国科学院遗传与发育生物学研究所 中国遗传学会
  • 主编:薛勇彪
  • 地址:北京市安定门外大屯路中科院遗传发育所
  • 邮编:100101
  • 邮箱:ycxb@genetics.ac.cn
  • 电话:010-64807669
  • 国际标准刊号:ISSN:1673-8527
  • 国内统一刊号:ISSN:11-5450/R
  • 邮发代号:2-819
  • 获奖情况:
  • 1996年获中科院优秀期刊二等奖,1997年获全国优秀期刊三等奖,200年获中科院优秀期刊二等奖
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,荷兰文摘与引文数据库,荷兰医学文摘,美国生物医学检索系统,美国科学引文索引(扩展库),美国生物科学数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2000版)
  • 被引量:17519