中文摘要：

目的：探讨微dxRNA-141（miR-141）对小鼠肝癌细胞株hepal-6高迁移率族蛋白B1（highmobilitygroupproteinB1，HMGBl）表达的调控作用。方法：将小鼠肝癌细胞株hepal-6分为miR-141拟似物转染组、miR-141拟似物转染对照组、miR-141抑制剂转染组、miR-141抑制剂转染对照组。分别用脂质体lipofectamine2000将miR-141转染hepal-6细胞。用实时定量PCR（quantitativereal—timePCR，qRT-PCR）检测miR-141和HMGBlmRNA表达，然后用Western印迹检测HMGBl蛋白表达。采用双荧光素酶报告基因检测miR-141对靶基因HMGBl的调控作用。结果：与相应对照组比较，miR-141拟似物转染组中miR-141表达水平上调，而miR-141抑制剂转染组中miR-141表达水平下调。同时，与相应对照组比较，miR-141拟似物转染组中HMGBlmRNA和蛋白表达明显下调，而miR-141抑制剂转染组中HMGBlmRNA和蛋白表达均明显上调。双荧光素酶报告基因分析表明miR-141能够作用于HMGBl基因的3＇-UTR。结论：miR-141可以作用于HMGBl基因的3＇-UTR，在转录后水平可上调HMGBl蛋白的表达，HMGBl可能是miR-141直接调控的靶基因。

英文摘要：

Objective： To explore the regulatory effect of microRNA-141 （miR-141） on expression of high mobility group protein B i （HMGB 1） in mouse hepatoma carcinoma cell line （hepal-6 cells）.Methods： qlae hepal-6 cells were divided into 4 groups： a miR-141 mimic group, a miR-141 mimic control group, a miR-141 inhibitor group and a miR-141 inhibitor control group, q-he miR-141 was transfected into hepal-6 cells with lipofectamine 2000. The levels of miR-141 and HMGB1 mRNA in the hepal-6 cells were detected by quantitative real-time PCR （qRT-PCR）, and then HMGB1 protein was examined by Western blot. The regulatory effect of miR-141 on 3＇-UTR of candidate target gene （HMGB i） was determined by dual-luciferase reporter assay. Results： Compared with the control group, the level of miR-141 was up-regulated in the miR- 141 mimic group, while down-regulated in the miR-141 inhibitor group. Moreover, the levels of HMGB1 mRNA and protein decreased in the miR-141 mimic group, while increased in the miR- 141 inhibitor group.Ihe dual-luciferase reporter assay showed that miR-141 could target the 3＇-UTR ofHMGB1 gene. Conclusion： MiR-141 can up-regulate the expression of HMGB1 protein at the post- transcriptional level by targeting to the specific sequence of 3＇-UTR of HMGB1 gene, which suggests that HMGB1 gene maybe a target gene ofmiR-141.