中文摘要：

精确地评估繁体中文药(TCM ) 的功效是困难的，它导致剂量效果分析的无常和复杂性。在这研究，我们建立了 biomarkers 的焦点模式描绘 Gegen Qinlian 煎(GQD ) 的剂量效果关系，为对待类型 2 糖尿病 mellitus (2-DM ) 的一个 TCM 公式。2-DM 的一个老鼠模型通过喂的饮食与低剂量的 STZ 注射相结合的高脂肪被建立。有 2-DM 的老鼠是管理高度，中间或低剂量(6.785， 4.071， 1.357 mgl/L ) 在 vitro 引起了 glomerular podocyte apoptosis，它被无毒的 chrysin (1-20 mol/L ) dose-dependently 通过 DNA 的减小稀释破碎。Chrysin 处理 dose-dependently 恢复了增加的 Bax/Bcl-2 比率，并且在高暴露葡萄糖的肾的 podocytes 压制了 Apaf-1 正式就职和提高的细胞色素 c 版本。在糖尿病的 db/db 老鼠， chrysin 的口头的管理(10 mgf 1.84 ??? ？？

英文摘要：

It is difficult to accurately evaluate the efficacy of traditional Chinese medicine （TCM）, which leads to the uncertainty and complexity of dose-effect analysis. In this study we established the ＂Focus＂ mode of biomarkers to characterize the dose-effect relationship of Gegen Qinlian Decoction （GQD）, a TCM formula for treating type 2 diabetes mellitus （2-DM）. A rat model of 2-DM was established through high fat diet feeding combined with low-dose STZ injection. Rats with 2-DM were administered high, middle or low doses （6.785, 4.071, 1.357 mg·kg^-1·d^-1, respectively） of GQD extract for 60 d. Metformin （300 mg·kg^-1·d^-1） was taken as the positive control. Blood samples were collected to assess serum biochemical indexes and metabolic profiling. After ＂Focus＂ analysis, the biochemical index triglycerides （TG） and insulin sensitivity （ISl） were identified as focused integrated biomarkers （FIBs）, while arachidonic acid and docosatetraenoic acid were the metabolic FIBs. Dose-effect relationship curves of GQD were built based on these types of FIBs. Furthermore, the two dose-effect relationship curves showed similar trends with the middle dosage displaying the greatest efficacy, suggesting that insulin function and arachidonic acid metabolism played important roles in 2-DM and the responses to GQD. The metabolic FIB docosatetraenoie should be further explored for understanding its involvement in the process of 2-DM occurrence and the treatment. This ＂Focus＂ mode provides a novel strategy to evaluate the dose-effect relationship of a TCM. The system and concepts established here may also be applicable for assessing the dose-effect relationships of Western medicines.