中文摘要：

在本实验中我们用优化的免疫荧光化学法结合激光共聚焦显微技术，观察了微丝在小鼠卵细胞不同期的分布情况及PKB／Akt对小鼠卵母细胞和早期胚胎的微丝聚合的影响。结果显示，在小鼠卵母细胞及早期胚胎中均有微丝的表达，且主要集中在纺锤体处的质膜处、极体及分裂沟处。注射激活型PKB／AktmRNA能够增强微丝的聚集。相反，注射激酶失活型的PKB／AktmRNA减弱了微丝的聚合。因而我们认为PKB／Akt可以影响小鼠卵细胞和早期胚胎的微丝聚集。

英文摘要：

In the present study we aimed at elucidating the polymerization of the actin in mouse oocytes and embryos after treated with different kinase active mRNA of PKB. We observed the expression of actin and the polymerization after treated with PKB mRNA in the mouse oocytes and embryos by using modified immunofluorescent staining and laser confocal microscopy. The results showed that the actin was expressed in mouse oocytes and embryos mostly at spindle, polar body and contractile ring. Injection of mRNA coding for a constitytively active myristoylated PKB/Akt into oocytes or embryos induced polymerization of actin, whereas microinjection of mRNA of kinse-deficient PKB/Akt inhibited the formation of actin storage. So our findings confirmed that PKB activation was necessary for polymerization of actin in mouse oocytes and early embryos.