棉花(Gossypium hirsutum ) ,世界范围的最重要的纺织品庄稼,经常在它的生长期间遇到象干旱和涝灾那样的不能生活的应力季节(夏天) ,和它的生产率被不利因素显著地限制。为了调查这的分子的改编机制,种种类到不能生活的应力,编码血浆膜的基因内在的蛋白质(果仁) 在棉花被孤立,并且指定了为 GhPIP2; 7。量的反向的 transcriptase 聚合酶链反应分析显示了那 GhPIP2; 7 优先地在子叶和叶子被表示,并且它的表示在在干旱处理以后的叶子是起来调整的。GhPIP2 驾驶的 GUS 基因的强壮的表示; 7 倡导者在 5- 的叶子被检测到 10-day-old 当幼苗进一步发展了,转基因的 Arabidopsis 幼苗,而是 GUS 活动逐渐地变得弱。GhPIP2; 7 倡导者活动被甘露糖醇处理显著地也导致。而且,酵母房间过去表示的 GhPIP2; 7 显示的相对更高的干旱忍耐,与控制相比。GhPIP2 的在表示上; 7 在提高的 Arabidopsis 种公差到干旱应力。一起,这些数据建议了那 GhPIP2; 7 基因可以涉及叶开发并且响应干旱应力。
Cotton (Gossypium hirsutum), the most important textile crop worldwide, often encounters abiotic stress such as drought and waterlog during its growth season (summer), and its productivity is significantly limited by adverse factors. To investigate the molecular adaptation mechanisms of this plant species to abiotic stress, a gene encoding the plasma membrane intrinsic protein (PIP) was isolated in cotton, and designated as GhPIP2;7. Quantitative reverse transcriptase polymerase chain reaction analysis indicated that GhPIP2;7 was preferentially expressed in cotyledons and leaves, and its expression was up-regulated in leaves after drought treatments. Strong expression of GUS gene driven by GhPIP2;7 promoter was detected in leaves of 5- to 10-day-old transgenic Arabidopsis seedlings, but GUS activity gradually became weak as the seedlings further developed. GhPIP2;7 promoter activity was also remarkably induced by mannitoi treatment. Furthermore, yeast cells over-expressing GhPIP2;7 displayed relatively hi~her drought tolerance, comuared with controls. Over-expression of GhPIP2;7 in Arabidopsis enhanced plant tolerance to drought stress. Collectively, these data suggested that GhPIP2;7 gene may be involved in leaf development and in response to drought stress.