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A cotton gene encoding a plasma membrane aquaporin is involved in seedling development and in response to drought stress
  • ISSN号:1672-9145
  • 期刊名称:《生物化学与生物物理学报:英文版》
  • 分类:S718.521.2[农业科学—林学] Q753[生物学—分子生物学]
  • 作者机构:[1]Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China
  • 相关基金:This work was supported by grants from the National Natural Science Foundation of China (No. 31000529, 31070281) and the Ministry of Agriculture of China for Transgenic Research (No. 2011ZX08009-003).
中文摘要:

棉花(Gossypium hirsutum ) ,世界范围的最重要的纺织品庄稼,经常在它的生长期间遇到象干旱和涝灾那样的不能生活的应力季节(夏天) ,和它的生产率被不利因素显著地限制。为了调查这的分子的改编机制,种种类到不能生活的应力,编码血浆膜的基因内在的蛋白质(果仁) 在棉花被孤立,并且指定了为 GhPIP2; 7。量的反向的 transcriptase 聚合酶链反应分析显示了那 GhPIP2; 7 优先地在子叶和叶子被表示,并且它的表示在在干旱处理以后的叶子是起来调整的。GhPIP2 驾驶的 GUS 基因的强壮的表示; 7 倡导者在 5- 的叶子被检测到 10-day-old 当幼苗进一步发展了,转基因的 Arabidopsis 幼苗,而是 GUS 活动逐渐地变得弱。GhPIP2; 7 倡导者活动被甘露糖醇处理显著地也导致。而且,酵母房间过去表示的 GhPIP2; 7 显示的相对更高的干旱忍耐,与控制相比。GhPIP2 的在表示上; 7 在提高的 Arabidopsis 种公差到干旱应力。一起,这些数据建议了那 GhPIP2; 7 基因可以涉及叶开发并且响应干旱应力。

英文摘要:

Cotton (Gossypium hirsutum), the most important textile crop worldwide, often encounters abiotic stress such as drought and waterlog during its growth season (summer), and its productivity is significantly limited by adverse factors. To investigate the molecular adaptation mechanisms of this plant species to abiotic stress, a gene encoding the plasma membrane intrinsic protein (PIP) was isolated in cotton, and designated as GhPIP2;7. Quantitative reverse transcriptase polymerase chain reaction analysis indicated that GhPIP2;7 was preferentially expressed in cotyledons and leaves, and its expression was up-regulated in leaves after drought treatments. Strong expression of GUS gene driven by GhPIP2;7 promoter was detected in leaves of 5- to 10-day-old transgenic Arabidopsis seedlings, but GUS activity gradually became weak as the seedlings further developed. GhPIP2;7 promoter activity was also remarkably induced by mannitoi treatment. Furthermore, yeast cells over-expressing GhPIP2;7 displayed relatively hi~her drought tolerance, comuared with controls. Over-expression of GhPIP2;7 in Arabidopsis enhanced plant tolerance to drought stress. Collectively, these data suggested that GhPIP2;7 gene may be involved in leaf development and in response to drought stress.

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期刊信息
  • 《生物化学与生物物理学报:英文版》
  • 北大核心期刊(2004版)
  • 主管单位:
  • 主办单位:中国科学院上海生物化学研究所
  • 主编:
  • 地址:上海岳阳路319号
  • 邮编:200031
  • 邮箱:abbs@sibs.ac.cn
  • 电话:021-54920956 54920955
  • 国际标准刊号:ISSN:1672-9145
  • 国内统一刊号:ISSN:31-1940/Q
  • 邮发代号:4-210
  • 获奖情况:
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  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,荷兰文摘与引文数据库,美国生物医学检索系统,美国剑桥科学文摘,美国科学引文索引(扩展库),美国生物科学数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),英国英国皇家化学学会文摘,中国北大核心期刊(2000版)
  • 被引量:5851