中文摘要：

目的：利用高速逆流色谱法对酸枣仁黄酮类成分进行分离研究。方法：以乙酸乙酯-正丁醇-水（3∶2∶5）为溶剂系统,流动相的流速为1.0 mL.min-1,主机转速为1500 r.min-1,检测波长360 nm,对酸枣仁中黄酮类化合物进行分离;利用HPLC法测定化合物的纯度;利用ESI-MS及参照文献确定了化合物的结构。结果：首次从酸枣仁粗提物中分离得到：酸枣仁黄酮碳苷、6-芥子酰酸枣仁黄酮碳苷及6-阿魏酰酸枣仁黄酮碳苷,应用HPLC检测,纯度均在90%以上。结论：利用高速逆流色谱法分离酸枣仁中黄酮类化合物,快速,简单,重复性好,分离样品纯度高。

英文摘要：

Objective：To separate and isolate high-purity flavonoids from extract of Semen Ziziphi Spinosae by a preparative high-speed counter-current chromatography（HSCCC）.Methods：The solvent system was composed of ethyl acetate-n-butyl alcohol-water（3∶2∶5）,the flow rate was 1.0 mL·min-1,the rotational speed was 1500 r·min-1,and the detection wavelength was set at 360 nm.To separate flavonoids from Semen Ziziphi Spinosae;their purities were examined by HPLC;the structures of these compounds were identified by ESI-MS compared with the literatures.Results：Three flavonoids,spinosin,6-sinapoylspinosin and 6-feruloylspinosin were separated from crude extract of Semen Ziziphi Spinosae for the first time.The purities of obtained flavonoids were assessed to be over 90% by HPLC.Conclusion：HSCCC was proved to be a very fast and efficient tool for separation of high-purity flavonoids from Semen Ziziphi Spinosae,and the method is quick,simple,good repeatability and high purity.