中文摘要：

目的：探讨大肠杆菌表达的人源可溶性B淋巴细胞激活因子hsBAFF对小鼠脾脏B淋巴细胞免疫反应及其对胞内游离Ca^2＋信号变化的影响。方法：选择20只健康ICR小鼠，雌雄各半，随机分成两组（n=10）：①对照组；②hsBAFF实验组。实验组小鼠腹腔注射含hsBAFF（0．1mg／kgbw）的PBS溶液，对照组注射同等剂量的PBS溶液．连续8d。用MTT法检测小鼠脾脏B淋巴细胞的增殖及其对LPS刺激的免疫反应，并用激光共聚焦显微镜分析脾脏B淋巴细胞胞内钙离子水平（[Ca^2＋]i）变化。结果：hsBAFF注射小鼠的脾脏B淋巴细胞增殖和对LPS刺激的免疫反应均明显高于对照组（P〈0．05）；hsBAFF注射组[Ca^2＋]i荧光强度维持在相对稳定的高水平上波动，平均荧光强度显著高于对照组（P〈0．01），且荧光强度变化率小于对照组。结论：大肠杆菌表达的hsBAFF能促进B淋巴细胞的增殖和免疫应答，从而增强机体免疫功能。hsBAFF激活小鼠脾脏B淋巴细胞可能与[Ca^2＋]i升高有关。

英文摘要：

To investigate effects of hsBAFF synthesized in Escherichia coli on spleen B lymphocyte immune response and its intracellular free Ca^2 ＋ （[ Ca^2 ＋ ]i） signaling in mice. Methods： Twenty ICR mice, half males-half females, were chosen and randomly divided into a normal control group（ n= 10） and a hsBAFF treatment group（ n= 10）. The mice in hsBAFF treatment group were given abdominal cavity injection of hsBAFF ,solution which was diluted with phosphate buffered ,saline（PBS） at dosage of 0.1 mg/kg body weight once each day for over eight days. The mice in control group were received abdominal injection of PBS at the same dose and frequency. Spleen B lymphocyte proliferation and its immune response to LPS stimulation in mice were evaluated using an MTT assay, and change of spleen B lymphocyte[Ca^2＋ ]i was assayed under a laser scanning confocal microscope. Results： B lymphocyte pro- liferation and its immune response to LPS stimulation were significantly higher in hsBAFF-treated mice than in control mice （P 〈 0.05）. The B lymphocyte [ Ca^2 ＋ ]i fluorescence intensity in hsBAFF-treated mice maintained at a relatively high level fluctuation, and its average intensity was significantly higher to that of control mice （P 〈 0.01 ）, but change rate of the intensity was lower compared to that of control group. Conclusion： hsBAFF synthesized in Escherichia coli can enhance immune function in the body by increasing B lymphocyte proliferation and its immune response, hsBAFF-activated B lymphocyte function may be associated with in creasing B lymphocytes[ Ca^2＋ ]i.