中文摘要：

目的：研究碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）、成纤维细胞生长因子受体1（fibroblast growth factor receptor 1，FGFR1）及bFGF mRNA三者与肿瘤细胞（神经胶质瘤细胞）恶性行为的相关性。方法：设计、合成bFGF反义寡核苷酸，转染SWO-38神经胶质瘤细胞，用原位杂交、免疫组化及图像分析、间接ELISA、细胞增殖MTT法、细胞集落形成等方法，分别检测转染前后bFGF、FGFR1、bFGF mRNA表达的改变，观察不同剂量bFGF反义寡核苷酸转染后细胞生长、集落形成的变化。结果：大部分细胞bFGF mRNA和细胞生长可被bFGF反义寡核苷酸抑制，细胞生长的抑制呈浓度依赖性，最高抑制率可达到48％，其抑制作用可被外源性bFGF完全逆转；反义寡核苷酸处理组大部分细胞表达的FGFR1减少、细胞分泌的bFGF明显减少，集落形成抑制率达35％．外源性bFGF的逆转作用为8％。正义组和阴性对照组均无以上的明显改变。结论：揭示了反义寡核苷酸可特异性抑制bFGF mRNA和肿瘤细胞的bFGF合成表达，但不能拮抗外源性bFGF结合bFGFR的生物学作用；FGFR1随肿瘤细胞SWO-38中bFGF表达水平的降低而下调。

英文摘要：

Objective：To clarify the contribution of endogenous bFGF, bFGF mRNA and FGFR1 to the abnormal growth and phenotypic transformation of neoplastic tumors cells. Methods： The antisense oligonucleotide primers was used to evaluate the influence of endogenous bFGF on growth of human glioma malignant cell lines SWO-38 in vitro. MTr was used to examine the variety of ceils growth treated with bFGF antisense oligonucleotide primers. The methods of ELISA, in situ hybridization, immuno-hischemistry and image analysis were used to detect the expression level of bFGF, bFGF mRNA and FGFR1. The colony formation of cells in soft agar was used to assess the cloning efficiency of the ceils after exposed to bFGF antisense oligo-nucleotide primers. Results：The cells multiplication, expression of bFGF mRNA and FGFR1 was inhibited by bFGF antisense oligonucleotide primers, and the ceils multiplication was dose-dependent. Treated with antisense oligo-nucleotide primers, the expression of FGFR1 and secretion of bFGF were distinctly reduced, and the inhibition efficiency of Ceils multiplication of WSO-38 was 48% and the inhibition efficiency of colonies of SWO-38 in soft agar was 35%. The inhibition of ceils multiplication can be reversed completely by external bFGF, and the reverse efficiency was 8%. Conclusion：The synthesis of bFGF mRNA and expression of bFGF can be specifically inhibited by antisense oligonucleotide, but the inhibition can be cleared up with the addition of external bFGF. The study suggested that the bFGF？ antisense oligonucleotide could have good effect in inhibiting of tumor under special condition.