中文摘要：

目的：探讨短时授精对IVF受精正常患者体外受精-胚胎移植（IVF-ET）治疗结局的影响。方法：回顾性分析IVF受精率〉60％患者的临床资料，按授精方案分为短时授精组（n=153）和常规受精组伽=180）。结果：IVF短时授精组和常规IVF组分别获卵1619枚和l986枚，分别有133名和152名患者接受了新鲜胚胎移植。组间女方年龄、基础FSH（bFSH）、bLH、bE2、Gn量、hCG注射日FSH水平、hCG注射日E2水平、hCG注射日LH水平、获卵数、卵子成熟度及移植日内膜厚度均无统计学差异。IVF短时授精组和常规IVF组的正常受精率（65．55％ vs 67．84％）、正常卵裂率（98．02％ vs 96．90％）、胚胎种植率（29．41％ vs 827．84％、临床妊娠率（44．36％ vs 45．39％）、继续妊娠率（41．35％ vs 42．76％）、流产率（5．45％ vs 2．90％）和异位妊娠率（1．82％vs 2．90％）均无统计学差异，但是短时授精组的多原核受精率（14．56％）显著高于常规IVF组（6．19％），而短时授精组的优质胚胎率（33．51％）高于常规IVF组（28．99％）。结论：因IVF短时授精可避免常规IVF完全受精失败而逐渐在国内广泛应用，但其远期影响还有待进一步认识。

英文摘要：

Objective： To investigate the clinical significance of short co-incubation of gametes in patients with no barriers to fertilization during IVF-ET. Methods： IVF patients with fertilization rate 〉60% were collected in the study and divided into short co-incubations group （n=153） and traditional IVF group （n=180）. The parameters and clinical outcomes had been analyzed between above two groups. Results： The patients in short co-incubation group and traditional IVF group received 1 619 eggs and 1 986 eggs, and 133 patients and 152 patients accepted embryo-transfer in fresh cycles, respectively. There were no significant differences between the two groups in ages, basal FSH/LH/E_2 level, FSH/LH/E_2 level on hCG injection day, total dosage of Gn used, numbers of oocytes retrieved, maturation of oocyte and endometrial thickness in ET day. The rates of normal fertilization （65.55% vs 67.84%）, cleavage （98.02% vs 96.90%）, implantation （29.41% vs 27.84%）, clinical pregnancy （44.36% vs 45.39%）, ongoing pregnancy （41.35% vs 42.76%）, abortion （5.45% vs 2.90%） and ectopic pregnancy （1.82% vs 2.90%） between the two groups were not significantly different. However, polyspermy rate was significantly higher in short co-incubation group （14.56%） than in traditional IVF group （6.19%）, and short co-incubation group （33.51%） had more good-quality embryos than traditional IVF group （28.99%）. Conclusion： Short co-incubation of gametes combined with early rescue ICSI had been used widely for avoiding complete fertilization failure in traditional IVF. Long-term effects and indications of short co-incubation followed by early removal of granular cells should be further recognized and standardized.