中文摘要：

目的研究慢病毒在感染细胞后，是否全部整合入染色体。方法用慢病毒载体的假病毒感染293T细胞后．抽提培养2d和10d的细胞基因组DNA。通过定量PCR检测细胞基因组中LTR拷贝数，计算出整合率。分别取病毒感染3d和11d的细胞，离心重悬后取部分细胞放在荧光显微镜下拍照，在蛋白水平上验证此结论。结果①慢病毒载体在宿主细胞基因组内的平均整合率（47±16）％（n=10）；②细胞感染慢病毒后3d和11d的GFP照片也证实了慢病毒在宿主细胞基因组内部分整合。结论慢病毒载体的假病毒感染293T细胞后，仅有部分的载体骨架是整合人基因组中的，整合入293T细胞基因组中的外源基因能够较长时间稳定表达。

英文摘要：

Objective To study how much infected Lentiviral Vectors are integrated into the host cell genome. Methods The lentiviral pro-viral copy numbers in the infected 293T cells chro- mosome was determined by quantitative PCR analysis. The ratio of the integration on day 10 over day 2 infection was used to determine the integration rate. Moreover, day 11 and day 3 infection pictures were used to prove the conclusion. Results The integration rate on average was ： 47% ±0. 16 （n = 10） ; The GFP pictures taken on day 3 and day 11 proved such a conclusion. Conclusion Only part of infected Lentiviral Vectors are integrated into the host cell genome, and the integrated trans- gene could relatively stable express its transgene.